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Over Expression of CD44V8—10 in Human Bladder Cancer Cells Decreases Their Interaction With Hyaluronic Acid and Potentiates Their Malignant Progression

2004; Lippincott Williams & Wilkins; Volume: 171; Issue: 1 Linguagem: Inglês

10.1097/01.ju.0000093446.54115.b6

1527-3792

Mototsugu Muramaki, Hideaki Miyake, Sadao Kamidono, Isao Hara,

Fibroblast Growth Factor Research

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jan 2004Over Expression of CD44V8—10 in Human Bladder Cancer Cells Decreases Their Interaction With Hyaluronic Acid and Potentiates Their Malignant Progression MOTOTSUGU MURAMAKI, HIDEAKI MIYAKE, SADAO KAMIDONO, and ISAO HARA MOTOTSUGU MURAMAKIMOTOTSUGU MURAMAKI , HIDEAKI MIYAKEHIDEAKI MIYAKE , SADAO KAMIDONOSADAO KAMIDONO , and ISAO HARAISAO HARA View All Author Informationhttps://doi.org/10.1097/01.ju.0000093446.54115.b6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We evaluated the effects of the over expression of CD44v8–10, one of the high molecular form of CD44, on the malignant potential of UM-UC-3 human bladder cancer cells in vitro and in vivo. Materials and Methods: We introduced CD44v8–10 complementary DNA into UM-UC-3 cells, which do not express detectable level of CD44v8–10 protein, and generated the CD44v8–10 over expressing cell line UM-UC-3/v8–10 and the vector only transfected cell line UM-UC-3/C. The effects of the introduction of CD44v8–10 into UM-UC-3 cells on the ability to bind hyaluronic acid (HA) were analyzed by the cell adhesion assay and the cell migration assay. We then evaluated tumor progression of UM-UC-3 sublines after subcutaneous and orthotopic injection into athymic nude mice. Results: There were no significant differences in in vitro growth rates. UM-UC-3/v8–10 cells showed significantly decreased binding and migration ability to HA but not to other extracellular matrix proteins. Furthermore, UM-UC-3/v8–10 cells demonstrated significantly increased tumor growth after subcutaneous as well as orthotopic injection into athymic nude mice and enhanced lymph node metastasis after orthotopic injection compared with UM-UC-3/C cells. Conclusions: These findings suggest that CD44v8–10 over expression in human bladder cancer cells decreases their interaction with HA and potentiates their malignant progression. References 1 : Natural history of bladder cancer. Prognostic features and long-term disease course. Urol Clin North Am1992; 19: 429. Google Scholar 2 : Recent advances in bladder cancer chemotherapy. Cancer Invest2001; 19: 77. Google Scholar 3 : Interaction between CD44 and hyaluronate is directly implicated in the regulation of tumor development. J Exp Med1994; 180: 53. Google Scholar 4 : Expression of CD44R1 adhesion molecule in colon carcinoma and metastases. Lancet1993; 341: 725. 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Google Scholar From the Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe and Department of Urology, Hyogo Medical Center for Adults, Akashi, Japan© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 171Issue 1January 2004Page: 426-430 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Keywordsbladder neoplasmsbladderhyaluronic acidantigens, CD44mice, nudeMetricsAuthor Information MOTOTSUGU MURAMAKI More articles by this author HIDEAKI MIYAKE More articles by this author SADAO KAMIDONO More articles by this author ISAO HARA More articles by this author Expand All Advertisement PDF downloadLoading ...