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Tyrosine Phosphorylation of p85 Relieves Its Inhibitory Activity on Phosphatidylinositol 3-Kinase

2001; Elsevier BV; Volume: 276; Issue: 29 Linguagem: Inglês

10.1074/jbc.m100556200

1083-351X

Bruce D. Cuevas, Yiling Lu, Muling Mao, Jinyi Zhang, Ruth LaPushin, Kathy Siminovitch, Gordon B. Mills,

Cancer-related Molecular Pathways

Under resting conditions, the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) serves to both stabilize and inactivate the p110 catalytic subunit. The inhibitory activity of p85 is relieved by occupancy of the NH2-terminal SH2 domain of p85 by phosphorylated tyrosine. Src family kinases phosphorylate tyrosine 688 in p85, a process that we have shown to be reversed by the activity of the p85-associated SH2 domain-containing phosphatase SHP1. We demonstrate that phosphorylation of the downstream PI3K target Akt is increased in cells lacking SHP1, implicating phosphorylation of p85 in the regulation of PI3K activity. Furthermore, the in vitrospecific activity of PI3K associated with tyrosine- phosphorylated p85 is higher than that associated with nonphosphorylated p85. Expression of wild-type p85 inhibits PI3K enzyme activity as indicated by PI3K- dependent Akt phosphorylation. The inhibitory activity of p85 is accentuated by mutation of tyrosine 688 to alanine and reversed by mutation of tyrosine 688 to aspartic acid, changes that block and mimic tyrosine phosphorylation, respectively Strikingly, mutation of tyrosine 688 to aspartic acid completely reverses the inhibitory activity of p85 on cell viability and activation of the downstream targets Akt and NFκB, indicative of the physiological relevance of p85 phosphorylation. Tyrosine phosphorylation of Tyr688 or mutation of tyrosine 688 to aspartic acid is sufficient to allow binding to the NH2-terminal SH2 domain of p85. Thus an intramolecular interaction between phosphorylated Tyr688 and the NH2-terminal SH2 domain of p85 can relieve the inhibitory activity of p85 on p110. Taken together, the data indicate that phosphorylation of Tyr688in p85 leads to a novel mechanism of PI3K regulation. Under resting conditions, the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) serves to both stabilize and inactivate the p110 catalytic subunit. The inhibitory activity of p85 is relieved by occupancy of the NH2-terminal SH2 domain of p85 by phosphorylated tyrosine. Src family kinases phosphorylate tyrosine 688 in p85, a process that we have shown to be reversed by the activity of the p85-associated SH2 domain-containing phosphatase SHP1. We demonstrate that phosphorylation of the downstream PI3K target Akt is increased in cells lacking SHP1, implicating phosphorylation of p85 in the regulation of PI3K activity. Furthermore, the in vitrospecific activity of PI3K associated with tyrosine- phosphorylated p85 is higher than that associated with nonphosphorylated p85. Expression of wild-type p85 inhibits PI3K enzyme activity as indicated by PI3K- dependent Akt phosphorylation. The inhibitory activity of p85 is accentuated by mutation of tyrosine 688 to alanine and reversed by mutation of tyrosine 688 to aspartic acid, changes that block and mimic tyrosine phosphorylation, respectively Strikingly, mutation of tyrosine 688 to aspartic acid completely reverses the inhibitory activity of p85 on cell viability and activation of the downstream targets Akt and NFκB, indicative of the physiological relevance of p85 phosphorylation. Tyrosine phosphorylation of Tyr688 or mutation of tyrosine 688 to aspartic acid is sufficient to allow binding to the NH2-terminal SH2 domain of p85. Thus an intramolecular interaction between phosphorylated Tyr688 and the NH2-terminal SH2 domain of p85 can relieve the inhibitory activity of p85 on p110. Taken together, the data indicate that phosphorylation of Tyr688in p85 leads to a novel mechanism of PI3K regulation. phosphatidylinositol 3-kinase Src-homology interleukin hemagglutinin polyacrylamide gel electrophoresis 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide epidermal growth factor glutathioneS-transferase The PI3K1 signaling cascade has been linked to proliferation, cell survival, differentiation, apoptosis, cytoskeletal rearrangement, and vacuolar trafficking. Growth factor-responsive Class IA PI3Ks consist of heterodimers of a 110-kDa catalytic subunit associated with an 85-kDa noncatalytic regulatory subunit designated p85. The p85 adapter subunits are encoded by at least three different genes with splice variation generating multiple proteins potentially serving many different functions (1Fruman D.A. Snapper S.B. Yballe C.M. Davidson L., Yu, J.Y. Alt F.W. Cantley L.C. Science. 1999; 283: 393-397Crossref PubMed Scopus (572) Google Scholar). Of the known p85 adapter subunits and splice variants, nearly all contain two Src-homology 2 (SH2) domains, which enable p85 to bind phosphotyrosine in an appropriate amino acid context. The p85 SH2 domains most frequently, but not exclusively, recognize phosphotyrosine embedded in a YXXM motif (2Songyang Z. Shoelson S.E. McGlade J. Olivier P. Pawson T. Bustelo X.R. Barbacid M. Sabe H. Hanafusa H. Yi T. Ren R. Baltimore D. Ratnofsky S. Feldman R.A. Cantley L.C. Mol. Cell. Biol. 1994; 14: 2777-2785Crossref PubMed Scopus (834) Google Scholar). Most p85 gene products also include a Src homology 3 (SH3) domain, as well as other domains involved in protein-protein interactions (3Wymann M.P. Pirola L. Biochim. Biophys. Acta. 1998; 1436: 127-150Crossref PubMed Scopus (579) Google Scholar). All p85 family members contain a p110-binding motif located between the two SH2 domains. The diversity of protein interaction domains found among p85 family members likely contributes to the ability of multiple signaling proteins and pathways to activate PI3K. Under resting conditions, p85 serves to both stabilize p110 protein and inhibit PI3K lipid kinase activity, thereby increasing the amount of inert p110 available for activation (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar). This inhibitory effect is alleviated by binding of the SH2 domains of p85, and in particular the NH2-terminal SH2 domain, to tyrosine-phosphorylated peptides, as well as tyrosine-phosphorylated receptors or linker molecules containing the YXXM motif (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar). Tyrosine phosphorylation of p85 binding sites within growth factor receptor cytoplasmic domains and linker molecules thus results in the recruitment of p85 to the cell membrane with consequent release of p85-mediated inhibition of PI3K (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar) and colocalization of PI3K with its substrate membrane phosphatidylinositols (5Rameh L.E. Cantley L.C. J. Biol. Chem. 1999; 274: 8347-8350Abstract Full Text Full Text PDF PubMed Scopus (852) Google Scholar) and other regulatory molecules (6Blalock W.L. Weinstein-Oppenheimer C. Chang F. Hoyle P.E. Wang X.Y. Algate P.A. Franklin R.A. Oberhaus S.M. Steelman L.S. McCubrey J.A. Leukemia (Baltimore). 1999; 13: 1109-1166Crossref PubMed Scopus (147) Google Scholar, 7Mayer B.J. Gupta R. Curr. Top. Microbiol. Immunol. 1998; 228: 1-22PubMed Google Scholar). The regulatory p85 subunit of PI3K is phosphorylated at tyrosine 688 (Tyr688) by the Src family kinases Lck and Abl (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar) and dephosphorylated at this site by the protein tyrosine phosphatase, SHP-1 (9Cuevas B. Lu Y. Watt S. Kumar R. Zhang J. Siminovitch K.A. Mills G.B. J. Biol. Chem. 1999; 274: 27583-27589Abstract Full Text Full Text PDF PubMed Scopus (131) Google Scholar). While p85 is known to be tyrosine-phosphorylated in response to a variety of stimuli, the role of p85 tyrosine phosphorylation in PI3K activation is unknown (6Blalock W.L. Weinstein-Oppenheimer C. Chang F. Hoyle P.E. Wang X.Y. Algate P.A. Franklin R.A. Oberhaus S.M. Steelman L.S. McCubrey J.A. Leukemia (Baltimore). 1999; 13: 1109-1166Crossref PubMed Scopus (147) Google Scholar, 10Yu Z. Su L. Hoglinger O. Jaramillo M.L. Banville D. Shen S.H. J. Biol. Chem. 1998; 273: 3687-3694Abstract Full Text Full Text PDF PubMed Scopus (81) Google Scholar, 11von Willebrand M. Baier G. Couture C. Burn P. Mustelin T. Eur. J. Immunol. 1994; 24: 234-238Crossref PubMed Scopus (40) Google Scholar). Tyrosine phosphorylation of p85 does, however, correlate with proliferative rate in Jurkat cells (12Martinez-Lorenzo M.J. Anel A. Moneleon I. Sierra J.J. Pineiro A. Naval J. Alava M.A. Int. J. Cell Biol. 2000; 32: 435-445Crossref PubMed Scopus (33) Google Scholar) and alters SH2 domain binding properties (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). Previous data from our group have revealed that coexpression of a constitutively active form of Lck with PI3K in COS cells results in an increase in PI3K activity (9Cuevas B. Lu Y. Watt S. Kumar R. Zhang J. Siminovitch K.A. Mills G.B. J. Biol. Chem. 1999; 274: 27583-27589Abstract Full Text Full Text PDF PubMed Scopus (131) Google Scholar). In this system, coincident expression of SHP-1 is associated with a decrease in PI3K activity, while expression of a phosphatase-inactive form of SHP-1 increases PI3K activity. These data suggest that phosphorylation/dephosphorylation of Tyr688, a residue that maps within the p85 carboxyl SH2 domain, provides a mechanism for regulating PI3K activity. The data described herein directly address this latter possibility and demonstrate that tyrosine phosphorylation of p85 and, more specifically of Tyr688, regulates PI3K activity, NFκB activation, and growth factor deprivation-induced cell death. The data also link these effects of Tyr688 phosphorylation to the formation of an intramolecular complex with the p85 NH2-terminal domain relieving the inhibitory effect of p85 on p110. The anti-phosphotyrosine monoclonal antibody (4G10, IgG2B) and the rabbit polyclonal antibody against the p85 subunit of PI3K were purchased from Upstate Biotechnology (Lake Placid, NY). The rabbit polyclonal antibodies against Akt and phospho-Akt were purchased from New England Biolabs (Beverly, MA). A monoclonal antibody against hemagglutinin (12CA5, IgG1) was purified from cell culture supernatants of the hybridoma provided by Dr. Bing Su (University of Texas M. D. Anderson Cancer Center, Houston, TX). Horseradish peroxidase-goat anti-mouse IgG was purchased from Bio-Rad. The cDNA plasmid for activated Lck Y505F was a generous gift of Dr. A. Villette (Montreal, Quebec, Canada). The cDNA plasmids for HAAkt, and HAp85 were generous gifts of Dr. Rakesh Kumar (University of Texas M. D. Anderson Cancer Center, Houston, TX). The cDNA plasmid for HACSH2 was a generous gift of Dr. Tomas Mustelin (Laboratory of Signal Transduction, La Jolla Cancer Research Center, The Burnham Institute, La Jolla, CA), and the cDNA plasmid for pGL3/NFκB was a generous gift of Dr. David Spencer (Baylor College of Medicine, Houston, TX). COS7 cells were purchased from American Type Culture Collection (Manassas, VA). Baf/3 was a kind gift of Dr. Tada Taniguchi (University of Tokyo, Tokyo, Japan). Baf/3, MDA MB 468, and COS7 cells were cultured in RPMI 1640 medium (Life Technologies, Inc.) containing penicillin/streptomycin (1%, Life Technologies, Inc.),l-glutamine (2 mm, Life Technologies, Inc.), and 10% (v/v) fetal calf serum (Sigma) at 37 °C in a humidified atmosphere. IL-3-producing cells were purchased from American Type Culture Collection (Manassas, VA). Human epidermal growth factor was purchased from Sigma. After stimulation, the cells were pelleted, resuspended in 0.5 ml of lysis buffer (150 mm NaCl, 50 mm Hepes, pH 7.4, 1 mm sodium orthovanadate, 50 mm ZnCl2, 50 mm NaF, 50 mm sodium orthophosphate, 2 mm EDTA, 2 mm phenylmethylsulfonyl fluoride, and 1% Nonidet P-40) and incubated at 4 °C for 20 min. After centrifugation at 14,000 ×g for 5 min at 4 °C, postnuclear detergent cell lysates were collected. Plasmid cDNA was mutated using the QuikChange Mutagenesis kit (Stratagene, La Jolla, CA) as per the manufacturer's guidelines. All mutations were confirmed by sequencing. Adherent cells were transfected by lipofection. Briefly, 4 × 105 cells were seeded on 100-mm cell culture plates and incubated in complete medium overnight. cDNA expression constructs were incubated in serum-free medium with LipofectAMINE (Life Technologies, Inc.) at room temperature for 30 min, then diluted with serum-free medium and incubated with cells at 37 °C for 2 h, after which time the LipofectAMINE mixture was replaced with complete medium, and the cells were returned to 37 °C for 24 h. Complete medium was then removed, the cells rinsed, and incubation continued with serum-free medium for an additional 24 h. Baf/3 cells were transfected by electroporation at 250 V and 950 microfarads. Detergent cell lysates were incubated with the appropriate antibody as indicated (anti-HA, anti-p85) at 4 °C for 2 h followed by another 2-h incubation with protein A-Sepharose beads. The immunoprecipitates were washed with immunoprecipitation wash buffer (0.5% Triton X-100, 150 mm NaCl, 10 mm Tris, pH 7.4, 1 mmEDTA, 1 mm EGTA, 0.2 mm sodium vanadate, 0.2 mm phenylmethylsulfonyl fluoride, and 0.5% Nonidet P-40). Proteins were eluted from the beads by boiling in 2× Laemmli buffer and separated by SDS-PAGE. Proteins were transferred to Immobilon (Millipore, Bedford, MA). Membranes were blocked in 3% bovine serum albumin and incubated with anti-p85 PI3K (1:1000), or anti-phosphotyrosine (1:3000), at room temperature for 2 h. Horseradish peroxidase-protein A or horseradish peroxidase-goat anti-mouse IgG was used as a secondary reagent. After extensive washing, the targeted proteins were detected by enhanced chemiluminescence (ECL). Where indicated, blots were stripped by treatment with 2% SDS and 100 mm β-mercaptoethanol in Tris-buffered saline and then reprobed with desired antibodies and detected by ECL. Cells were lysed in 1% Nonidet P-40 lysis buffer. Cell lysates normalized for protein levels were immunoprecipitated using anti-HA or anti-p85 and protein A-Sepharose. Nontransfected COS7 lysate immunoprecipitates were included as a negative control. PI3K activity was determined as described previously (9Cuevas B. Lu Y. Watt S. Kumar R. Zhang J. Siminovitch K.A. Mills G.B. J. Biol. Chem. 1999; 274: 27583-27589Abstract Full Text Full Text PDF PubMed Scopus (131) Google Scholar). To evaluate Akt phosphorylation, single cell suspensions of thymocytes were prepared from 2–3-week-old C3HeBFeJ motheaten (me) and wild-type control littermates derived from C3HeBFeJ me/+ breeding pairs maintained at the Samuel Lunenfeld Research Institute, Mount Sinai Hospital (Toronto, Ontario, Canada). For analysis of Akt phosphorylation, 5 × 107 thymocytes were resuspended in 100 µl of RPMI 1640 medium and incubated for 30 min at 4 °C with biotinylated anti-T cell antigen receptor antibody (1 µg/ml) or anti-CD3 (5 µg/ml) plus anti-CD28 (5 µg/ml) antibody. After removal of unbound antibody, cells were resuspended at a concentration of 2 × 108 cell/ml and incubated at 37 °C for 2 min with streptavidin (10 µg/ml) or anti-hamster IgG (10 µg/ml). After antibody stimulation, cells were pelleted by 0.5-min centrifugation and then lysed and analyzed as described above. Baf/3 cells were washed and reconstituted in complete medium at 11.25 × 106 cells/ml, mixed with the desired DNA, and electroporated. Samples were cultured in IL-3-free conditions for 48 h, then IL-3 was added to the sample, and the culture was continued for an additional 48 h. Triplicate 100-µl samples of each culture were transferred to a 96-well plate, mixed with 25 µl of MTT (5 mg/ml), and incubated at 37°C for 2 h. The samples were lysed with 100 µl of MTT lysis buffer (20% SDS in 50% N,N-dimethylformamide, pH 4.7) and absorbance at 570 nm determined. The luciferase assay kit was purchased from Promega (Madison, WI), and assays were carried out according to manufacturer's recommendations. We have previously demonstrated that SHP1 associates with PI3K and dephosphorylates the kinase at tyrosine 688 (9Cuevas B. Lu Y. Watt S. Kumar R. Zhang J. Siminovitch K.A. Mills G.B. J. Biol. Chem. 1999; 274: 27583-27589Abstract Full Text Full Text PDF PubMed Scopus (131) Google Scholar), a residue that maps within the p85 subunit and that has been shown to be phosphorylated by Src family kinases (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). These data indicate that SHP-1 modulates p85 tyrosine phosphorylation and raise the possibility that signal transduction through the PI3K pathway can be modulated by the SHP-1 tyrosine phosphatase. To investigate this possibility, resting and T cell antigen receptor-stimulated thymocytes from SHP-1-deficient motheaten mice were evaluated for PI3K activation using an assay of Akt Ser473 phosphorylation as a surrogate indicator of PI3K activity. As indicated in Fig.1, results of immunoblotting analysis revealed the level of Akt Ser473 phosphorylation induced in T cell antigen receptor-stimulated thymocytes to be markedly higher in motheaten compared with wild-type cells. These data indicate a role for SHP-1 in regulating not only p85 phosphorylation but also the activation of PI3K. By extension, these findings imply that the tyrosine phosphorylation status of p85 is relevant to the regulation of PI3K activity. To determine whether tyrosine phosphorylation of p85 alters the specific activity of PI3K, p85 activity was evaluated in either COS7 cells or COS7 cells transiently transfected with Lck Y505F, a constitutively active form of Lck that phosphorylates p85 at Tyr688 (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). To evaluate PI3K activity in relation to phosphorylation status, anti-p85 immunoprecipitates were prepared from either cell lysates subjected to preclearing with anti-phosphotyrosine antibody (i.e. lysates immunodepleted of tyrosine-phosphorylated species) or alternatively from anti-phosphotyrosine immunoprecipitates (so as to isolate tyrosine phosphorylated p85 species). The amount of p85 present in each sample was determined by Western blotting, and equal amounts of p85 were then assessed for lipid kinase activity using phosphatidylinositol as a substrate. As indicated in Fig. 2, this analysis revealed the enzymatic activity of p110 associated with tyrosine phosphorylated p85 (i.e. the p85 present in anti-Tyr(P) immunoprecipitates) to be much greater than that associated with p85 immunoprecipitated from cell lysates precleared with anti-phosphotyrosine antibody. These results suggest that PI3K lipid kinase activity is increased in association with p85 tyrosine phosphorylation and therefore provide additional evidence that PI3K activity is regulated by tyrosine phosphorylation. The tyrosine residue at position 688 has previously been identified as the primary site of Lck-induced p85 phosphorylation (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). To evaluate the impact of phosphorylation at this site on PI3K activity, p85 expression constructs were derived in which Tyr688 was replaced by either an aspartate or an alanine residue. Due to the charged nature of aspartate, p85 Y688D protein would be predicted to mimic phosphorylated p85 protein; by contrast, Y688A cannot be phosphorylated and should therefore behave like nonphosphorylated p85 (13Wu Y. Spencer S.D. Lasky L.A. J. Biol. Chem. 1998; 273: 5765-5770Abstract Full Text Full Text PDF PubMed Scopus (114) Google Scholar, 14Huang W. Erikson R.L. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 8960-8963Crossref PubMed Scopus (133) Google Scholar). As indicated in Fig.3 A, expression of these proteins in COS7 cells or MDA MB 468 cells revealed that the p85 Y688D and the Y688A mutant proteins were not tyrosine-phosphorylated either as a consequence of Lck coexpression or activation of cells with epidermal growth factor (EGF). These data thus confirm that Tyr688 is the primary site of tyrosine phosphorylation in p85. To further address the relevance of Tyr688phosphorylation to PI3K activation, the effects of these mutant proteins on Akt Ser473 phosphorylation was also assessed. As illustrated in Fig. 3 B, overexpression of wild-type p85 was associated with a decrease in Akt phosphorylation, a result consistent with the putative inhibitory effects of native (i.e. nonphosphorylated p85) on p110 kinase activity (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar). This effect was further accentuated in cells expressing the tyrosine nonphosphorylatable Y688A mutant protein (Fig. 3 B). By contrast, expression of the Y688D protein did not alter Akt phosphorylation, a result which implies that this protein facilitates p110 activation most likely by releasing the enzyme from p85-mediated inhibition. Conversion of Tyr688 to Ala or Asp did not alter association of p85 with p110 in the presence or absence of Lck (Fig. 3, B and C). Furthermore, Y688A and Y688D both associated equally with Cbl in the presence or absence of Lck (not presented), indicating that association with Cbl does not explain the differential effects of Y688A and Y688D on Akt phosphorylation. The effects of PI3K on cell survival are mediated at least in part by activation of Akt and the consequent phosphorylation and inactivation of pro-apoptotic proteins such as BAD (15del Peso L. Gonzalez-Garcia M. Page C. Herrera R. Nunez G. Science. 1997; 278: 687-689Crossref PubMed Scopus (1987) Google Scholar), GSK3 (16Pap M. Cooper G.M. J. Biol. Chem. 1998; 273: 19929-19932Abstract Full Text Full Text PDF PubMed Scopus (955) Google Scholar), forkhead (17Brunet A. Bonni A. Zigmond M.J. Lin M.Z. Juo P. Hu L.S. Anderson M.J. Arden K.C. Blenis J. Greenberg M.E. Cell. 1999; 96: 857-868Abstract Full Text Full Text PDF PubMed Scopus (5434) Google Scholar), and Caspase 9 (18Cardone M.H. Roy N. Stennicke H.R. Salvesen G.S. Franke T.F. Stanbridge E. Frisch S. Reed J.C. Science. 1998; 282: 1318-1321Crossref PubMed Scopus (2735) Google Scholar). To assess the relevance of p85 Tyr688 phosphorylation to these cellular events, the effects of wild-type and mutant p85 expression on cytokine deprivation-induced cell death were investigated using Baf/3, a cell line that undergoes apoptosis when cultured in the absence of IL-3 (19Leverrier Y. Thomas J. Perkins G.R. Mangeney M. Collins M.K. Marvel J. Oncogene. 1997; 14: 425-430Crossref PubMed Scopus (59) Google Scholar). For these studies, the IL-3-dependent Baf/3 cells were transfected with the various mutant cDNAs and then were cultured in IL-3-free medium for 48 h to induce cytokine deprivation-induced apoptosis and for an additional 48 h with exogenous IL-3 to allow surviving cells to proliferate. This provides a more sensitive assay than assessing cell number following growth factor deprivation. As shown in Fig. 4, expression of wild-type and Y688A p85 in these cells was associated with their decreased survival as compared with cells expressing vector control. Although both wild-type and Y688A induced a decrease in cell survival, the wild-type protein inhibited survival more consistently than did Y688A (Fig. 3 B). In contrast, survival of cells expressing the Y688D mutant protein was not significantly different from that of vector control cells. Thus the expression of wild-type or Y688A p85 protein appears to inhibit PI3K activity and induce a decrease in cellular proliferation/survival, while expression of the Y688D protein has a negligible effect on cell survival. These data are therefore consistent with the contention that p85 phosphorylation modulates PI3K function and also with the capacity for aspartic acid substitution at position Tyr688 to disrupt p85 inhibitory effects on p110 activity. To further address the functional significance of p85 phosphorylation, the effects of the various p85 mutant proteins on NFκB-directed transcription events were next evaluated. This approach was based on data revealing that activated Akt phosphorylates the IKKβ complex, resulting in the phosphorylation and consequent ubiquitination and degradation of the NFκB inhibitor IκB (20Karin, M., and Ben-Neriah, Y. Annu. Rev. Immunol., 18, 621–663.Google Scholar). Dissociation from IκB allows NFκB to translocate to the nucleus and participate in the formation of functional transcription complexes (20Karin, M., and Ben-Neriah, Y. Annu. Rev. Immunol., 18, 621–663.Google Scholar). Accordingly, the ability of the p85 mutant proteins to alter transcription of an NFκB-driven luciferase reporter construct was used as another measure of their effects on PI3K activation. As illustrated in Fig. 5, an assessment of COS7 cells transfectants expressing Akt and either p85 wild-type, Y688A, or Y688D proteins revealed that both wild-type and Y688A p85 significantly inhibited transcription of the NFκB consensus promoter (p < 0.05). In contrast, the Y688D mutant p85 did not inhibit NFκB-driven luciferase production. These data, which imply that Tyr688 phosphorylation status modulates transcription through NFκB, are again consistent with the notion that p85 phosphorylation regulates p110 enzymatic activity. Interaction of phosphotyrosine with the NH2-terminal SH2 domain of p85 has been shown to relieve the inhibitory activity of p85 on p110 (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar). As phosphorylation of p85 Y688 appears to have this same effect on p110, it is possible that phosphorylated Tyr688 interacts with the NH2-terminal p85 SH2 domain so as to generate a structural arrangement that counteracts the inhibitory effects of this domain. Although Tyr688 does not map within the consensus binding motif for the p85 NH2-terminal SH2 domain (YXXM), previous data have identified the capacity of nonconsensus sequences to bind to the p85 subunit (21Ponzetto C. Bardelli A. Maina F. Longati P. Panayotou G. Dhand R. Waterfield M.D. Comoglio P.M. Mol. Cell. Biol. 1993; 13: 4600-4608Crossref PubMed Scopus (159) Google Scholar, 22He T.C. Zhuang H. Jiang N. Waterfield M.D. Wojchowski D.M. Blood. 1993; 82: 3530-3538Crossref PubMed Google Scholar). Moreover, this type of intramolecular interaction might be facilitated by a p85 structural conformation that positions the carboxyl terminus including Tyr688 in close proximity to the NH2-terminal SH2 domain of p85, a possibility predicted by previous molecular modeling data (3Wymann M.P. Pirola L. Biochim. Biophys. Acta. 1998; 1436: 127-150Crossref PubMed Scopus (579) Google Scholar). Such an arrangement might evoke interaction of the NH2-terminal SH2 domain with the tyrosine-phosphorylated Tyr688 residue within the COOH-terminal SH2 domain. To begin addressing this possibility, the potential for p85 tyrosine phosphorylation to promote an interaction between Tyr688and the p85 NH2-terminal SH2 domain was studied using transfected COS7 cells coexpressing Lck Y505F and an epitope-tagged p85 carboxyl-terminal fragment (HACT) encompassing the Tyr688residue. The results of this analysis confirmed that Lck induces tyrosine phosphorylation of the HACT construct and revealed the capacity of endogenous p85 to coimmunoprecipitate with the tyrosine-phosphorylated p85 HACT domain but not the nonphosphorylated construct (Fig. 6 A). The data shown in Fig. 6 also demonstrate the capacity of a glutathioneS-transferase (GST)-linked amino-terminal SH2 domain fusion protein (NSH2-GST) to precipitate phosphorylated HACT (Fig.6 A) and HACT Y688D proteins but not a nonphosphorylated HACT construct. Together, these findings suggest that the amino-terminal SH2 domain of p85 associates with tyrosine-phosphorylated Tyr688. As shown in Fig. 6 B, mutation of tyrosine 688 to aspartate (to mimic tyrosine phosphorylation) allows the association between HACT and NSH2-GST protein to ensue in the absence of Lck Y505F, an observation that confirms the involvement of phosphorylated Tyr688 in this association and rules out the possibility that Lck Y505F acts as a linker in coupling these domains together. The biochemical events governing protein tyrosine phosphorylation are central to the regulation of cellular signaling in all eukaryotic cells. However, while a myriad of intracellular proteins undergo tyrosine phosphorylation following cell stimulation, for many proteins, the effects of phosphorylation on function are not well defined. This latter group of proteins includes PI3K, an enzyme that is inducibly tyrosine-phosphorylated in many biological contexts. It has been suggested that PI3K is negatively regulated by serine autophosphorylation of the p85 regulatory subunit (23Dhand R. Hiles I. Panayotou G. Roche S. Fry M.J. Gout I. Totty N.F. Truong O. Vicendo P. Yonezawa K. Kasuga M. Courtneidge S.A. Waterfield M.D. EMBO J. 1994; 13: 522-533Crossref PubMed Scopus (415) Google Scholar). However, interaction of the p85 SH2 domains with tyrosine-phosphorylated peptides appears to alleviate this inhibition, a finding that implies a role for tyrosine phosphorylation in regulating PI3K activity (4Yu J. Zhang Y. McIlroy J. Rordorf-Nikolic T. Orr G.A. Backer J.M. Mol. Cell. Biol. 1998; 18: 1379-1387Crossref PubMed Google Scholar). This possibility is strongly supported by the current data showing that SHP-1, an enzyme that dephosphorylates the major tyrosine phosphorylation site on p85, Tyr688, down-regulates the PI3K/Akt activation pathway. Moreover, the current data, revealing lipid kinase activity to be higher in the p85 protein present in anti-phosphotyrosine immunoprecipitates than in the p85 protein immunoprecipitated from cell lysates immunodepleted for tyrosine phosphorylated species, also indicate a direct relationship between p85 phosphorylation status and PI3K activity. Enhanced PI3K activity in this latter experiment implies that the inhibitory effect of the p85 SH2 domains on enzymatic activity has been released, a phenomenon that might relate to the tyrosine phosphorylation of p85 per seor, alternatively, to interactions of the p85 SH2 domains with tyrosine-phosphorylated proteins captured by anti-phosphotyrosine immunoprecipitation. To distinguish between these possibilities, p85 proteins mutated at the major tyrosine phosphorylation site (Tyr688) were investigated with respect to their effects on PI3K activity. The results of this analysis revealed p85 Y688A protein, which cannot be phosphorylated at Tyr688, to be associated with impaired PI3K activity as manifested by decreases in Akt phosphorylation, BAF/3 cell survival, and NFκB promoter activation. By contrast, these latter activities were all enhanced in cells expressing a mutant p85 protein, Y688D, which is predicted to mimic tyrosine-phosphorylated p85. Taken together, these data provide compelling evidence that PI3K activity is regulated by phosphorylation of p85 at position Tyr688. While the crystal structure of full-length p85 bound to phosphopeptide is not currently available, the predicted protein sequence of the intervening iSH2 domain (p110 binding site between the two SH2 domains) indicates a pair of antiparallel helices and thus predicts that the two SH2 domains are closely aligned (3Wymann M.P. Pirola L. Biochim. Biophys. Acta. 1998; 1436: 127-150Crossref PubMed Scopus (579) Google Scholar). These data raise the possibility of an intramolecular association involving binding of the phosphorylated Tyr688 residue within the p85 carboxyl-terminal tail to the p85 amino-terminal SH2 domain. This model, which is illustrated in Fig. 7, is supported by the current data, which reveal the ability of full-length p85 to associate with the phosphorylated, but not nonphosphorylated, Tyr688-containing carboxyl-terminal fragment of p85 and which also suggest that this association is mediated via the p85 amino-terminal SH2 domain (Fig. 6). The data also exclude the possibility that this association depends upon Lck functioning as an intermediary “linker” protein, as the association occurs in the absence of Lck when p85 Y688D is used in the analysis. Together, these data suggest the existence of an intramolecular interaction, between phosphorylated Tyr688 and the amino-terminal SH2 domain of p85 (Fig. 7). Although the amino acid sequence surrounding Tyr688 does not conform to the expected p85 SH2 target sequence (YXXM), this SH2 domain has already been shown to exhibit flexibility in terms of the target motif (21Ponzetto C. Bardelli A. Maina F. Longati P. Panayotou G. Dhand R. Waterfield M.D. Comoglio P.M. Mol. Cell. Biol. 1993; 13: 4600-4608Crossref PubMed Scopus (159) Google Scholar, 22He T.C. Zhuang H. Jiang N. Waterfield M.D. Wojchowski D.M. Blood. 1993; 82: 3530-3538Crossref PubMed Google Scholar). Furthermore, an intramolecular association of the nature proposed here may provide a mechanism to prevent binding of the p85 SH2 domains to low affinity substrates. This possibility is supported by previous data revealing p85 association with several phosphorylated proteins to be disrupted upon Tyr688 phosphorylation (8von Willebrand M. Williams S. Saxena M. Gilman J. Tailor P. Jascur T. Amarante-Mendes G.P. Green D.R. Mustelin T. J. Biol. Chem. 1998; 273: 3994-4000Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). The current data suggest that this latter observation may reflect competitive inhibition consequent to the formation of an intramolecular association. As with SH2 occupation by other phosphopeptides, this association would serve to “relax” the p85-mediated inhibition of p110 PI3K activity. In addition to this model, the current data might also be explained by another model wherein phosphorylation of Tyr688 triggers an intermolecular interaction between individual p85 proteins, again inducing disruption of the inhibitory activity of p85 (Fig. 7). In this alternative “PI3K concatamer” model, the recruitment of multiple PI3K molecules could represent a mechanism whereby the PI3K signaling cascade is amplified. It is possible that p85 intramolecular interactions also promote PI3K signal amplification by facilitating the removal of phosphorylated PI3K and thus freeing the receptor for subsequent association with a new PI3K. The newly detached, phosphorylated PI3K could then be dephosphorylated by SHP-1 and returned to a basal state, once again available for recruitment to a phosphorylated receptor. Alternatively, an induced intramolecular interaction may represent a mechanism by which PI3K is removed from activated growth factor receptors. Recent studies have shown that an intermolecular interaction also occurs between the p85 SH3 and proline-rich domains (24Harpur A.G. Layton M.J. Das P. Bottomley M.J. Panayotou G. Driscoll P.C. Waterfield M.D. J. Biol. Chem. 1999; 274: 12323-12332Abstract Full Text Full Text PDF PubMed Scopus (40) Google Scholar), a result which suggests that concatamers of p85 may play a role in forming multimeric interaction complexes. Whichever model proves valid, the capacity of Y688D to mimic the effect of Tyr688 phosphorylation implies that the minimal requirement for this association is phosphorylation at p85 Tyr688. Taken together, the data indicate that phosphorylation of 688 relieves the inhibitory activity of p85 on p110 and suggest that this effect is mediated by the association of phosphorylated tyrosine 688 with the NH2-terminal SH2 domain of p85. Thus intramolecular interactions with phosphorylation sites in p85 have the potential to contribute to the outcome of ligand activation of cells. We thank the DNA Core Sequencing Facility for sequencing the p85 Y688A, p85 Y688D, and HACT Y688D constructs.