SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
2009; Sociedad Chilena de Química; Volume: 54; Issue: 1 Linguagem: Inglês
10.4067/s0717-97072009000100014
ISSN0717-9707
AutoresC ROJO, María Javiera Álvarez-Figueroa, Marco Soto‐Arriaza, Álvaro Cañete, C. David Pessoa‐Mahana, Camilo López‐Alarcón,
Tópico(s)Antioxidant Activity and Oxidative Stress
ResumoThe scavenging activity of diclofenac was estimated by its capability to bleach ABTS radical cation, and ORAC (oxygen radical absorbance capacity) methodology.In addition, GC-MS technique was used to establish if the lactamic derivative of diclofenac was formed as final product in the interaction with peroxyl radicals.Diclofenac bleached ABTS radical cation in a concentration-dependent way.In ORAC assays, diclofenac inhibited the consumption of fluorescein and c-phycocyanin mediated by peroxyl radicals.An ORAC-fluorescein, and ORAC-c-phycocyanin value of 0.3, and 0.004 was estimated, respectively.From the downcurvature observed in Stern-Volmer-like plots, we postulated that the low protection of c-phycocyanin is related to secondary damage of c-phycocyanin by the secondary radical of diclofenac.By GC-MS studies only a 2,6-dichloroaniline derivative was detected as product after the reaction with peroxyl radicals.
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