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BIBTEX RIS

EXPANSION OF THE INNER MEMBRANE COMPARTMENT AND ITS RELATION TO MITOCHONDRIAL VOLUME AND ION TRANSPORT

1968; Rockefeller University Press; Volume: 39; Issue: 2 Linguagem: Inglês

10.1083/jcb.39.2.382

ISSN

1540-8140

Autores

Lester Packer, John M. Wrigglesworth, P. A. George Fortes, Berton C. Pressman,

Tópico(s)

ATP Synthase and ATPases Research

Resumo

Glutaraldehyde has been used to fix mitochondria undergoing rapid volume changes associated with energized ion transport under oscillatory state conditions and valinomycin-induced potassium uptake. Fixation was found to prevent structural changes which normally occur during ion accumulation or loss. By correlating packed volume measurements with electron microscopy, it is shown that changes in volume associated with ion movements reflect changes in the inner membrane compartment and that this compartment can be related to the sucrose inaccessible space. The method can therefore be used to accurately determine volume changes that arise from ion translocation.

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