Portal de Periódicos da CAPES

Relationships between the Correction of Mismatched Bases in DNA and Mutability

1979; Cold Spring Harbor Laboratory Press; Volume: 43; Linguagem: Inglês

10.1101/sqb.1979.043.01.151

1943-4456

R. Holliday, Patricia J. Pukkila, Jennifer Dickson, Ad Spanos, Vincent Murray,

Chemical Reaction Mechanisms

In heterozygous crosses, hybrid DNA formed during recombination at meiosis may contain mismatched base pairs. Their repair or correction will result in gene conversion, and failure of repair will be followed by postmeiotic segregation (pms). Evidence that repair of this type can occur comes largely from genetic experiments both in prokaryotes and eukaryotes (Spatz and Trautner 1970; Leblon 1972a,b; Nevers and Spatz 1975; White and Fox 1975; Wildenberg and Meselson 1975; Miller et al. 1976; Wagner and Meselson 1976; Yu-Sun et al. 1977). This indicates that repair can occur in either direction (i.e., to mutant or wild-type base pairs) and that it involves the degradation and resynthesis of part of one polynucleotide chain (Fogel and Mortimer 1969; Fincham and Holliday 1970; Leblon and Rossignol 1973). Little information is available about the biochemistry or genetic control of this type of repair.