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Induction of Secretion of IgM from Cells of the B Cell Line 38C-13 by Somatic Cell Hybridization

1979; American Association of Immunologists; Volume: 122; Issue: 6 Linguagem: Inglês

10.4049/jimmunol.122.6.2430

1550-6606

Zelig Eshhar, Cila Blatt, Yehudit Bergman, Joseph Haimovich,

Immune Cell Function and Interaction

Cells of the murine B cell line 38C-13 possess immunoglobulins of the IgM class on their surface but do not secrete them. Upon hybridization of 38C-13 cells with murine myeloma cells, hybridoma clones were obtained that secreted both pentameric IgM of 38C-13 origin and the myeloma protein. All hybridoma clones synthesized and secreted large amounts of homogeneous IgM with a half disappearance time of about 2 hr, typical of mature plasma cells. Concomitantly with the induction of IgM secretion, the hybridoma cells lost their surface IgM. The possibility of separate pathways for the synthesis of membrane and secreted IgM is discussed.