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The Buccaneer software for automated model building. 1. Tracing protein chains

2006; Wiley; Volume: 62; Issue: 9 Linguagem: Inglês

10.1107/s0907444906022116

1399-0047

Kevin Cowtan,

Mass Spectrometry Techniques and Applications

A new technique for the automated tracing of protein chains in experimental electron-density maps is described. The technique relies on the repeated application of an oriented electron-density likelihood target function to identify likely Cα positions. This function is applied both in the location of a few promising `seed' positions in the map and to grow those initial Cα positions into extended chain fragments. Techniques for assembling the chain fragments into an initial chain trace are discussed.