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New primers for promising single-copy genes in fungal phylogenetics and systematics

2009; National Herbarium of the Netherlands; Volume: 23; Issue: 1 Linguagem: Inglês

10.3767/003158509x470602

1878-9080

Imke Schmitt, Ana Crespo, Pradeep K. Divakar, Johnathon D. Fankhauser, E. Herman-Sackett, Klaus Kalb, Matthew P. Nelsen, Nathaniel Aaron Nelson, Eimy Rivas-Plata, A.D. Shimp, Todd J. Widhelm, H. Thorsten Lumbsch,

Yeasts and Rust Fungi Studies

Developing powerful phylogenetic markers is a key concern in fungal phylogenetics.Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota).Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class.We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi.The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify Saccharomycotina, Taphrinomycotina and Basidiomycota.