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Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond

2008; Microbiology Society; Volume: 58; Issue: 1 Linguagem: Inglês

10.1099/ijs.0.65217-0

1466-5034

Luciana Albuquerque, Igor Tiago, Marco Taborda, M. Fernanda Nobre, António Veríssimo, Milton S. da Costa,

Microbial Community Ecology and Physiology

A low-G+C, Gram-positive isolate, designated strain CVS-8T, was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35–37 °C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4–6 % (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1γ type with meso-diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15 : 0, 16 : 0, anteiso-15 : 0 and iso-16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8T represented a novel species of the genus Bacillus, the highest levels of sequence similarity (mean pairwise similarity values of ∼97.5 %) being found with respect to the type strains of Bacillus shackletonii and Bacillus acidicola. On the basis of the phylogenetic, physiological and biochemical data, strain CVS-8T represents a novel species of the genus Bacillus, for which the name Bacillus isabeliae sp. nov. is proposed. The type strain is CVS-8T (=LMG 22838T=CIP 108578T).