Characterization of DNA polymerase β mRNA: cell-cycle and growth response in cultured human cells
1988; Oxford University Press; Volume: 16; Issue: 20 Linguagem: Inglês
10.1093/nar/16.20.9587
ISSN1362-4962
AutoresBarbara Z. Zmudzka, A. Fomace, James M. Collins, Samuel H. Wilson,
Tópico(s)Meat and Animal Product Quality
ResumoDNA polymerase beta (beta-polymerase) is a housekeeping enzyme involved in DNA repair in vertebrate cells. We used a cDNA probe to study abundance of beta-polymerase mRNA in cultured human cells. The mRNA level in synchronized HeLa cells, representing different stages of the cell-cycle, varied only slightly. Contact inhibited fibroblasts AG-1522 contained the same level of mRNA as growing cells. The steady-state level of mRNA in fibroblasts is equivalent to 6 molecules per cell. The results indicate that the beta-polymerase transcript is "low abundance" and is neither cell-cycle nor growth phase responsive.
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