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Acute Exposure to Ozone Exacerbates Acetaminophen-Induced Liver Injury in Mice

2010; Oxford University Press; Volume: 115; Issue: 1 Linguagem: Inglês

10.1093/toxsci/kfq034

1096-6080

Daher Ibrahim Aibo, Neil Birmingham, Ryan P. Lewandowski, Jane F. Maddox, Robert A. Roth, Patricia E. Ganey, James G. Wagner, Jack R. Harkema,

Liver physiology and pathology

Ozone (O3), an oxidant air pollutant in photochemical smog, principally targets epithelial cells lining the respiratory tract. However, changes in gene expression have also been reported in livers of O3-exposed mice. The principal aim of the present study was to determine if acute exposure to environmentally relevant concentrations of O3 could cause exacerbation of drug-induced liver injury in mice. Overdose with acetaminophen (APAP) is the most common cause of drug-induced liver injury in developed countries. In the present study, we examined the hepatic effects of acute O3 exposure in mice pretreated with a hepatotoxic dose of APAP. C57BL/6 male mice were fasted overnight and then given APAP (300 mg/kg ip) or saline vehicle (0 mg/kg APAP). Two hours later, mice were exposed to 0, 0.25, or 0.5 ppm O3 for 6 h and then sacrificed 9 or 32 h after APAP administration (1 or 24 h after O3 exposure, respectively). Animals euthanized at 32 h were given 5-bromo-2-deoxyuridine 2 h before sacrifice to identify hepatocytes undergoing reparative DNA synthesis. Saline-treated mice exposed to either air or O3 had no liver injury. All APAP-treated mice developed marked centrilobular hepatocellular necrosis that increased in severity with time after APAP exposure. O3 exposure increased the severity of APAP-induced liver injury as indicated by an increase in necrotic hepatic tissue and plasma alanine aminotransferase activity. O3 also caused an increase in neutrophil accumulation in livers of APAP-treated animals. APAP induced a 10-fold increase in the number of bromodeoxyuridine-labeled hepatocytes that was markedly attenuated by O3 exposure. Gene expression analysis 9 h after APAP revealed differential expression of genes involved in inflammation, oxidative stress, and cellular regeneration in mice treated with APAP and O3 compared to APAP or O3 alone, providing some indications of the mechanisms behind the APAP and O3 potentiation. These results suggest that acute exposure to near ambient concentrations of this oxidant air pollutant may exacerbate drug-induced liver injury by delaying hepatic repair.