Artigo Revisado por pares

Platelet Clumping in vitro

1964; Wiley; Volume: 10; Issue: 1 Linguagem: Inglês

10.1111/j.1365-2141.1964.tb00681.x

ISSN

1365-2141

Autores

J.R.A. Mitchell, A. A. Sharp,

Tópico(s)

Hemophilia Treatment and Research

Resumo

O sler (1874), Hayem (1878) and Eberth and Schimmelbusch (1886) showed that the process of blood clotting initiated platelet clumping in shed blood, and later workers (Roskam, 1922; Zucker and Borrelli, 1955; Bergsagel, 1956; Bounameaux, 1957) have shown that various coagulation factors released during the clotting process can produce this platelet clumping. There has, however, been a growing body of evidence to suggest that other mechanisms, not directly related to clotting, could also initiate platelet clumping (Sharp, 1958, 1961), and the existence of one such mechanism was confirmed by the observation that a protein‐free, heat‐stable, dialysable extract of human red cells could increase platelet adhesiveness (Hellem, 1960). Hellem called the active principle of his extract ‘Factor R’ fractionation of the extract showed that the active agent was adenosine diphosphate (ADP) and it was found that when this substance was added to platelet‐rich citrated plasma in concentrations as low as 0.02 μg./ml., platelet clumping was produced (Gaarder, Jonsen, Laland, Hellem and Owren, 1961). We have studied this clumping phenomenon in vitro, tested other substances for clumping activity and searched for materials which would modify the process. This paper reports the results of these studies.

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