Endothelial cells preparing to die by apoptosis initiate a program of transcriptome and glycome regulation
2003; Wiley; Volume: 18; Issue: 1 Linguagem: Inglês
10.1096/fj.03-0097fje
ISSN1530-6860
AutoresNicola Johnson, Shiladitya Sengupta, Samir Saidi, Khashayar Lessan, D. Stephen Charnock‐Jones, Laurie Scott, Richard Stephens, Tom C. Freeman, Brian D. M. Tom, M. Scott Harris, Gareth Denyer, Mallik Sundaram, Ram Sasisekharan, S. K. Smith, Cristin G. Print,
Tópico(s)Cell Adhesion Molecules Research
ResumoThe protein-based changes that underlie the cell biology of apoptosis have been extensively studied. In contrast, mRNA- and polysaccharide-based changes have received relatively little attention. We have combined transcriptome and glycome analyses to show that apoptotic endothelial cell cultures undergo programmed changes to RNA transcript abundance and cell surface polysaccharide profiles. Although a few of the transcriptome changes were protective, most appeared to prepare cells for apoptosis by decreasing the reception and transduction of pro-survival signals, increasing pro-death signals, increasing abundance of apoptotic machinery, inhibiting cellular proliferation, recruiting phagocytes to regions of cell death, and promoting phagocytosis. Additional transcriptomal changes appeared to alter the synthesis and modification of cell surface glycosaminoglycans. The resultant reduced abundance of sulphated cell surface glycosaminoglycans may further promote cell death by inhibiting the presentation of extracellular matrix-tethered survival factors to their receptors on dying cells. We propose that the transcriptome and glycome regulation presented here synergize with previously described protein-based changes to guide the apoptotic program.
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