The signaling adapter p62 is an important mediator of T helper 2 cell function and allergic airway inflammation
2006; Springer Nature; Volume: 25; Issue: 15 Linguagem: Inglês
10.1038/sj.emboj.7601250
ISSN1460-2075
AutoresPilar Martı́n, Marı́a T. Diaz-Meco, Jorge Moscat,
Tópico(s)IL-33, ST2, and ILC Pathways
ResumoArticle27 July 2006free access The signaling adapter p62 is an important mediator of T helper 2 cell function and allergic airway inflammation Pilar Martin Pilar Martin Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Search for more papers by this author Maria T Diaz-Meco Maria T Diaz-Meco Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Department of Genome Science, Genome Research Institute, Cincinnati, OH, USA Present address: Department of Genome Science, Genome Research Institute, 2180 E Galbraith Road, Cincinnati, OH, 45237 USA Search for more papers by this author Jorge Moscat Corresponding Author Jorge Moscat Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Department of Genome Science, Genome Research Institute, Cincinnati, OH, USA Present address: Department of Genome Science, Genome Research Institute, 2180 E Galbraith Road, Cincinnati, OH, 45237 USA Search for more papers by this author Pilar Martin Pilar Martin Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Search for more papers by this author Maria T Diaz-Meco Maria T Diaz-Meco Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Department of Genome Science, Genome Research Institute, Cincinnati, OH, USA Present address: Department of Genome Science, Genome Research Institute, 2180 E Galbraith Road, Cincinnati, OH, 45237 USA Search for more papers by this author Jorge Moscat Corresponding Author Jorge Moscat Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain Department of Genome Science, Genome Research Institute, Cincinnati, OH, USA Present address: Department of Genome Science, Genome Research Institute, 2180 E Galbraith Road, Cincinnati, OH, 45237 USA Search for more papers by this author Author Information Pilar Martin1, Maria T Diaz-Meco1,2,3 and Jorge Moscat 1,2,3 1Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain 2Department of Genome Science, Genome Research Institute, Cincinnati, OH, USA 3Present address: Department of Genome Science, Genome Research Institute, 2180 E Galbraith Road, Cincinnati, OH, 45237 USA *Corresponding author. Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Canto Blanco, 28049 Madrid, Spain. Tel.: +34 91 497 8039; Fax: +34 91 761 6184; E-mail: [email protected] or [email protected] The EMBO Journal (2006)25:3524-3533https://doi.org/10.1038/sj.emboj.7601250 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Naïve T helper (Th) cells differentiate in response to antigen stimulation into either Th1 or Th2 effector cells, which are characterized by the secretion of different set of cytokines. Th2 differentiation, which is critical for allergic airway disease, is triggered by signals of the T-cell receptor (TCR) and the cytokines generated during polarization, particularly IL-4. We determine here the potential role of the signaling adapter p62 in T-cell polarization. We report using p62−/− mice and cells that p62 acts downstream TCR activation, and is important for Th2 polarization and asthma, playing a significant role in the control of the sustained activation of NF-κB and late synthesis of GATA3 and IL-4 by participating in the activation of the IKK complex. Introduction The abnormal activation and/or differentiation of CD4+ T lymphocytes along the T helper 2 cell (Th2) lineage underlie the pathology of asthma and other allergic diseases (Luster and Tager, 2004). Naïve Th cells differentiate in response to antigen stimulation into either Th1 or Th2 effector cells, which secrete different sets of cytokines and perform also different regulatory functions in the immune system (Mosmann and Coffman, 1989; Shuai and Liu, 2003). Th1 cells mainly produce IFN-γ and IL-2, and play an essential role in cell-mediated immune responses against intracellular pathogens. On the other hand, Th2 cells produce IL-4, IL-5, IL-10 and IL-13, and are important in the control of humoral immunity and allergy. Understanding the signaling pathways controlling Th2 differentiation is of paramount importance not only because it would lead to better therapies for asthma but also because it constitutes a paradigmatic model of cell differentiation in which a large number of signaling cascades have to crosstalk for an adequate control of the process (Paul and Seder, 1994). Th2 differentiation is modulated by signals emanating from the T-cell receptor (TCR) and the cytokines generated during polarization, particularly IL-4, which is added in the culture medium of CD4+ T cells induced to differentiate into the Th2 lineage in vitro (Ho and Glimcher, 2002; Murphy and Reiner, 2002). Therefore, IL-4 is important for induction and maintenance of differentiated Th2 cells. It shares with IL-13 interactions with the IL-4R chain and activates the transcription factor Stat6 through a Jak1/Jak3 signaling pathway (O'Shea et al, 2002). The crosstalk between signals generated by the TCR and IL-4 results in the physiological activation of T cells to induce and activate Th2-polarized T cells (Murphy and Reiner, 2002). Recently, we have found that the atypical PKC isoform PKCζ plays a critical role in Th2 differentiation and allergic airway inflammation, acting upstream Jak1 in the IL-4 signaling pathway (Martin et al, 2005). PKCζ and the other atypical PKC isofom PKCλ/ι harbor a PB1 domain at their N-terminal region, which interacts with other PB1 domain-containing proteins, such as p62 and Par-6, that act as scaffolds conferring specificity to the kinase's actions (Moscat and Diaz-Meco, 2002). Thus, the binding of the aPKCs with Par-6 serves to locate these enzymes in the polarity pathways, whereas the aPKC–p62 interaction is important for the sustained activation of NF-κB during osteoclast differentiation (Moscat and Diaz-Meco, 2002; Duran et al, 2004b). Since PKCζ is important during Th2 polarization and asthma, and because p62 is a scaffold of the aPKCs in several cells systems, we sought to determine here the potential role of p62 in these biological processes. We have found that, in marked contrast to the PKCζ−/− phenotype, the loss of p62 does not lead to an appreciable impairment of the IL-4 signaling pathway but plays an essential role downstream of TCR activation in the stimulation of the late and sustained NF-κB pathway, a required event for Th2 polarization and allergic airway inflammation. Results Role of p62 in the secretion of Th2 cytokines Since the loss of PKCζ results in impaired Th2 activation and asthma in mice, we reasoned that if p62 were the adapter of PKCζ in the IL-4 cascade, p62−/− mice should display defective Th2 polarization. To determine whether p62, like PKCζ, plays a role in lineage commitment of Th cells, CD4+ naïve T cells either wild-type (WT) or p62−/− were differentiated in vitro under Th1 or Th2 polarizing conditions, after which cells were stimulated with anti-CD3 plus anti-CD28 antibodies for 24 h, and the secretion of IFN-γ and IL-4 was determined in Th1 and Th2 cultures, respectively. Interestingly, whereas IFN-γ secretion is not affected (Figure 1A, left panel), IL-4 secretion is significantly reduced in p62−/− cells (Figure 1A, right panel). The synthesis of three other Th2 cytokines such as IL-5, IL-10 and IL-13 was also inhibited in p62−/− Th2 cells (Figure 1B). The levels of intracellular IL-4 were determined in the presence of Brefeldin A by FACS analysis in CD4+ T cells induced to differentiate into the Th2 lineage for 5 days, and afterwards re-stimulated with anti-CD3 for 5 h. From these experiments, it is clear that the percentage of WT Th2 producing IL-4 is significantly more than that of p62−/− Th2 cells (Figure 1C, upper panels), consistent with the ELISA data of Figure 1A and B. Together, these results suggest that p62 plays a non-redundant role in Th2-polarized CD4+ T cells. Surprisingly, when naïve T cells were treated in parallel under Th0 conditions for 5 days and re-stimulated for 5 h as above, the levels of intracellular IL-4 were also reduced in the p62−/− cell cultures as compared with the WT controls (Figure 1C, lower panels). This suggests that p62 is required for optimal production of IL-4 by naïve T cells when stimulated through the TCR under non-skewing conditions. To further support these observations, we next determined whether the loss of p62 would affect the secretion of IFN-γ, IL-4 or IL-2 by naïve CD4+ T cells activated by anti-CD3 plus anti-CD28. From these experiments, it is clear that there is a significant impairment in the secretion of IL-4 and IL-2 but not of IFN-γ in p62−/− cells as compared to the WT controls (Figure 2A and B). These observations will be consistent with the notion that p62, although not absolutely required, is essential for the initial steps of the Th2 differentiation process. As IL-2 has been shown to be important for Th2 differentiation and IL-4 secretion (Cote-Sierra et al, 2004), we next determined whether the exogenous addition of IL-2 to the culture medium of naïve CD4+ T cells stimulated with anti-CD3 plus anti-CD28 would correct the defect in IL-4 secretion observed in the p62-deficient T cells. Results of Figure 2C show that this is not the case, as the same reduced secretion of IL-4 is observed in the absence and in the presence of exogenous IL-2. Of note, the reduced IL-2 levels produced by the activated naïve p62−/− T cells do not have an appreciable role in proliferation (Supplementary Figure 1A and 1B) nor in early or late apoptosis (Supplementary Figure 1C and 1D) of the mutant T cells. Collectively, these results suggest that p62 participate in the control of late-event TCR-mediated signaling pathways important for IL-4 production and Th2 polarization. Figure 1.Role of p62 in the secretion and synthesis of Th2 cytokines. Naïve CD4+ T cells either WT or p62−/− were induced to differentiate for 5 days along the Th1 (A, left panel) or the Th2 (A, right panel, and B) lineages as described under Materials and methods, after which they were restimulated for 24 h with anti-CD3 (10 μg/ml) plus anti-CD28 (2 μg/ml) and the secretion of IFN-γ, IL-4, IL-5, IL-10 and IL-13 was determined by ELISA. (C) IL-4 intracellular staining in the presence of Brefeldin A (BD GolgiPlug, added 4 h before harvest) of Th2 or Th0 WT and p62-deficient (KO) cells treated with anti-CD3 (10 μg/ml) plus anti-CD28 (2 μg/ml) for 5 h. Inset numbers represent the percentage of IL-4-positive cells. The results in panels A and B are the mean±s.d. of three independent experiments with incubations in triplicate. The results in panel C are representative of another two experiments. Download figure Download PowerPoint Figure 2.Role of p62 in cytokine secretion of naïve T cells. Naïve CD4+ T cells were stimulated with anti-CD3 (10 μg/ml) plus anti-CD28 (2 μg/ml) for 72 h (A) or for different times (B), and secretion of IL-4 (A), IFN-γ (A) or IL-2 (B) was determined by ELISA. (C) Cells were stimulated as in panel A but in the presence or absence of exogenous IL-2 (10 ng/ml), and secretion of IL-4 was determined by ELISA. Results are the mean±s.d. of three independent experiments with incubations in triplicate. Download figure Download PowerPoint Impaired Th2 signaling in p62-deficient T cells To gain insights into the biochemical alterations produced during the Th2 polarization process by the loss of p62, we initially determined the level of the transcription factor GATA3, a very well-established hallmark of Th2 differentiation (Ho and Glimcher, 2002). Thus, naïve CD4+ T cells were polarized under either Th0, Th1 or Th2 skewing conditions, after which intracellular GATA3 levels were determined by flow cytometry using the appropriate antibody as described previously (Martin et al, 2005). Data of Figure 3A show that GATA3 is synthesized in WT CD4+ T cells induced to polarize under Th2 but not under Th0 or Th1 conditions. Importantly, the loss of p62 significantly reduces the percentage of Th2 lymphocytes synthesizing GATA3 (Figure 3A). When extracts prepared from these cell cultures were analyzed by immunoblotting, it was apparent that GATA3 levels were reduced in the p62−/− Th2 cells as compared to the WT controls (Figure 3B). It is well established that, in addition to the activation of the TCR, IL-4 has to be added to these cell cultures for Th2 polarization to occur. The tyrosine phosphorylation of Stat6 is a reliable marker of the activation of the IL-4 signaling cascade. When phospho-Stat6 levels were analyzed in the above cultures, it was apparent that the absence of p62 only very modestly reduced Stat6 phosphorylation, as well as that the phospho-Stat5 levels, a marker of IL-2 signaling, were not affected by the loss of p62 (Figure 3B). Taken together, these results suggest that, in contrast to the PKCζ−/− phenotype in which Stat6 phosphorylation was severely impaired in Th2-polarized T cells (Martin et al, 2005), p62 does not appear to be critical for the Stat6 pathway. In fact when naïve CD4+ T cells were stimulated with IL-4, the activation of Stat6 phosphorylation was not affected at all in the p62−/− cells (Figure 3D), again contrary to what has previously been observed in PKCζ−/− T cells (Martin et al, 2005). No differences in Stat6 phosphorylation were observed between WT and knockout (KO) cells even when stimulated with a lower dose of IL-4 (Figure 3D–F). Collectively, these results strongly suggest that p62 is important for optimal Th2 polarization but does not seem to be required for IL-4 signaling. In contrast, nuclear RelA (Figure 3B) as well as p50 (Figure 3C) levels were reduced in p62−/− Th2 cells as compared to the WT controls, indicating that p62 plays a significant role during NF-κB activation in Th2 differentiation. Interestingly, NF-κB has been shown to be essential for GATA3 synthesis (Das et al, 2001). Figure 3.Biochemical parameters of Th2 differentiation. (A) Intracellular staining of GATA3 in WT and p62−/− (KO) Th0, Th1 and Th2 cells differentiated for 5 days as described in Materials and methods. The solid line represents GATA3 staining; the dashed line corresponds to the mouse IgG1 isotype control. Inset numbers represent the percentage of GATA3-positive cells. (B) Immunoblot analysis of nuclear levels of GATA3, RelA, phospho-Stat6, phospho-Stat5 and PCNA in cell cultures of CD4+ T cells either WT and p62-deficient (KO) differentiated along the Th0, Th1 and Th2 pathways for 5 days as above; nuclear levels of p50 in Th2-polarized WT and p62−/− (KO) cells were determined by immunoblotting as in panel B. (C) Phospho-Stat6 levels of naïve CD4+ T cells stimulated with IL-4 (20 ng/ml) for different times (D, E) or with two concentrations of IL-4 for 20 min (F) were determined by immunoblotting. The results are representative of three independent experiments. Download figure Download PowerPoint Role of p62 in sustained NF-κB activation in T cells Th2 polarization is a long-term differentiation process that requires the sustained activation of TCR-derived signals. Since p62 has been shown previously in osteoclasts to play a critical role in the sustained, but not in the early, activation of NF-κB in that system (Duran et al, 2004b), we next determined here whether p62 was important for early and/or sustained T-cell activation. Thus, we first analyzed the impact that the loss of p62 has in early signaling events activated by the TCR. Our data demonstrate that the TCR proximal pathways such as the activation of Akt, ERK or ZAP-70 were not affected in p62−/− naïve CD4+ T cells triggered with anti-CD3 plus anti-CD28 (Figure 4A). Another marker of early T-cell activation is the intracellular Ca2+ level. Consistent with the lack of involvement of p62 in early T-cell signaling, intracellular Ca2+ levels increase similarly in WT and p62-deficient T cells activated with anti-CD3 (Figure 4B). Early NF-κB activation as determined by IκBα degradation was not inhibited in p62−/− T cells activated in response to anti-CD3 plus anti-CD28, whereas IκBβ degradation was not appreciably affected in either type of T cells (Figure 4C). These results indicate that p62 is not required for the activation of TCR-triggered early signaling events. Consistent with this, the phosphorylation of Bcl-10, which plays a critical role in the orchestration of the TCR-triggered signalosome complex (Huang and Wange, 2004), was not affected by the loss of p62 (not shown). Figure 4.TCR-activated signaling pathways. Naïve CD4+ T cells either WT or p62−/− were stimulated for different times with anti-CD3 (10 μg/ml) plus anti-CD28 (2 μg/ml) and the levels of different signaling molecules including p62 were determined by immunoblot analysis (A, C, D, F, G). Other cultures were differentiated for 5 days under Th0, Th1 or Th2 polarizing conditions, as above, and the levels of p62 and actin were determined by immunoblotting (E). Naïve CD4+ T cells were loaded with Fluo-3 and stimulated with anti-CD3 (20 μg/ml) crosslinking. Histograms represent calcium mobilization analyzed by flow cytometry (B). Naïve CD4+ T cells activated as above with anti-CD3 (10 μg/ml) plus anti-CD28 (2 μg/ml) for different times were analyzed by EMSA for NF-κB activation (H). Jurkat T cells were stimulated with PMA (50 ng/ml) plus ionomycin (50 ng/ml) for different times, after which Malt1 was immunoprecipitated and the associated p62 was detected by immunoblotting (I); control immunoprecipitation of the 24-h stimulated samples with either anti-Malt1 antibody or an irrelevant anti-HA antibody, followed by immunoblotting with anti-p62 and anti-Malt1 antibodies demonstrate the specificity of the p62–Malt1 interaction (J). The results are representative of three independent experiments. Download figure Download PowerPoint We have recently reported that p62 is induced during, and required for, late differentiation events in other cell systems (Duran et al, 2004b). In order to determine whether p62 is induced in Th cell differentiation, naïve CD4+ T cells were stimulated with anti-CD3 plus anti-CD28 and p62 levels were determined by immunoblotting at different times thereafter. Interestingly, p62 is dramatically induced in activated naïve T cells at 6 and 12 h post-stimulation (Figure 4D) and remained elevated up until 48 h, the maximum time measured (not shown). These results would be consistent with a model according to which p62 is induced in T cells committed to differentiate. The data of Figure 4E show that, even though p62 is detectably induced in T cells differentiating along the Th1 lineage, its synthesis is more potently activated in Th2-polarized T cells. These data, together with our results demonstrating that p62 is required for optimal Th2 differentiation and RelA activation in Th2-polarized cells (see above), suggest that p62 induction is a required event for late NF-κB signaling, GATA3 activation and subsequent T-cell polarization along the Th2 lineage. To further support this notion, naïve CD4+ T cells were stimulated with anti-CD3 plus anti-CD28 for 12 and 24 h, after which IκBα phosphorylation was determined by immunoblotting. Results of Figure 4F demonstrate that IκBα phosphorylation, a marker of NF-κB activity, was significantly inhibited in p62−/− T cells as compared to the WT controls. Consistently, phospho-IKK levels were robustly induced at 24 h post-activation of WT CD4+ naïve T cells but not in the p62-deficient ones (Figure 4G), indicating that p62 is required for late activation of IKK and NF-κB in naïve T cells. These results demonstrate that p62 is required for an optimal activation of NF-κB at later times in the differentiation process of CD4+ T cells, an event important for GATA3 induction and Th2 polarization. Results of Figure 4H confirm by EMSA the role of p62 in the sustained activation of NF-κB. The activation of NF-κB in T cells involves the formation of a signaling complex including three critical molecules, namely Carma1, Bcl-10 and Malt1 (Huang and Wange, 2004). Malt1 appears to be the most proximal to the IKK complex and its presence is absolutely required for the activation of NF-κB through a still not totally clarified mechanism (Ruefli-Brasse et al, 2003; Ruland et al, 2003; Sun et al, 2004; Zhou et al, 2004). In other cell systems in which p62 has been implicated in the activation of NF-κB, p62 has been shown to form part of the signaling complex that regulates IKK activation (Sanz et al, 2000; Duran et al, 2004b; Wooten et al, 2005). In order to determine whether this is also the case in T lymphocytes, we stimulated Jurkat T cells with PMA plus ionomycin for 30 min, or 16 and 24 h, after which Malt1 was immunoprecipitated and the potential association of p62 was determined by immunoblotting. Results of Figure 4I demonstrate that there is a basal association of p62 with Malt1 that is significantly induced at 16 and 24 h post-stimulation. However, at 30 min, the association of p62 with Malt1 did not differ from that seen in unstimulated cells (Figure 4I). Results of Figure 4J demonstrate that p62 co-immunoprecipitation with Malt1 is actually specific. These results are consistent with the concept that p62 is required for optimal activation of late but not early NF-κB and that p62 is directly or indirectly able to interact with Malt1. To better understand the details of this interaction, we first took into account that both p62 and Malt1 interact with TRAF6 (Sanz et al, 1999; Sun et al, 2004). Therefore, we transfected 293 cells with HA-p62 along with expression vectors for Flag-TRAF6 and Flag-Malt1 or empty plasmids. Afterwards, cell extracts were prepared, HA-p62 was immunoprecipitated and the associated Flag-TRAF6 and Flag-Malt1 was analyzed by immunoblotting with anti-Flag antibody. The results of Figure 5A confirm our previous observations that TRAF6 potently interacts with p62. In addition, although Malt1 weakly interacts with p62 under these conditions, this interaction is potently enhanced in the presence of TRAF6 (Figure 5A). These results would be in keeping with the notion that Malt1 binds p62 and that TRAF6 helps in the formation of a p62/TRAF6/Malt1 complex. Next, we stimulated Jurkat cells with PMA plus ionomycin for different times, after which the association of endogenous p62 with endogenous TRAF6 was determined. From the data of Figure 5B it is apparent that p62 constitutively binds TRAF6 in unstimulated and in stimulated cells. Collectively, these results suggest that the preformed p62/TRAF6 complex is recruited to Malt1 during late T-cell activation. Recent results from different investigators showed that Malt1, likely through the ability of TRAF6 to act as an E3 ubiquitin ligase, controls IKK activation most likely by inducing its K63 ubiquitination (Ruefli-Brasse et al, 2003; Ruland et al, 2003; Sun et al, 2004; Zhou et al, 2004). These observations, together with our recent finding that p62 plays an important role in regulating TRAF6 ubiquitinating activity (Wooten et al, 2005), led us to determine whether p62 induces TRAF6 to promote the ubiquitination of the IKK regulatory subunit IKKγ (also known as Nemo). Thus, 293 cells were transfected with HA-tagged ubiquitin and Myc-IKKγ either with or without His-p62 along with a plasmid control or Flag-TRAF6. Cell extracts were immunoprecipitated with anti-Myc and the ubiquitination of IKKγ was determined by immunoblotting with anti-HA. Interestingly, the relatively weak ubiquitination of IKKγ promoted by TRAF6 is dramatically enhanced in p62-cotransfected cells, whereas p62 alone did not detectably induce IKKγ ubiquitination (Figure 5C). To further understand the mechanism whereby p62 controls IKKγ ubiquitination, 293 cells were transfected with HA-p62 and Myc-IKKγ along with a control plasmid or Flag-TRAF6. HA-tagged p62 was immunoprecipitated and the associated Myc-IKKγ was determined by immunoblotting with anti-Myc. The results of Figure 5D demonstrate that TRAF6 favors the interaction of IKKγ with p62. Interestingly, data of Figure 5E demonstrate that endogenous IKKγ recruits endogenous p62 at 16 and 24 h post-stimulation of Jurkat T cells but not at early times (10 and 30 min). Therefore, all these results will be consistent with a model according to which the formation of a Malt1–TRAF6/p62–IKKγ complex at late time after T-cell induction serves to trigger the ubiquitination of IKKγ and the activation of IKK and the NF-κB pathway. Figure 5.Important role of p62 in IKKγ ubiquitination. Extracts from 293 cells transfected with different combinations of HA-p62, Flag-TRAF6 and Flag-Malt1 expression plasmids were immunoprecipitated with anti HA antibody and the immunoprecipitates were analyzed by immunoblotting with anti-Flag antibody (A). Jurkat T cells were stimulated for different times with PMA (50 ng/ml) plus ionomycin (50 ng/ml), after which extracts were prepared and endogenous TRAF6 was immunoprecipitated and the associated endogenous p62 was determined by immunoblotting with an anti-p62 antibody; specificity control of the immunoprecipitation was carried out with an irrelevant anti-HA antibody (B). 293 cells were transfected with HA-ubiquitin and Myc-IKKγ with or without combinations of His-p62 and Flag-TRAF6; extracts were prepared and immunoprecipitated with anti-Myc antibody and the ubiquitination of IKKγ was determined by immunoblotting with anti-HA antibody (C). 293 cells were transfected with HA-p62 and Myc-IKKγ along with a plasmid control or a Flag-TRAF6 expression vector, after which cell extracts were immunoprecipitated with anti-HA and the associated IKKγ was detected by immunoblotting with anti-Myc antibody (D). Jurkat T cells were stimulated for different times with PMA (50 ng/ml) plus ionomycin (50 ng/ml), after which extracts were prepared and endogenous IKKγ was immunoprecipitated and the associated endogenous p62 was determined by immunoblotting with an anti-p62 antibody; specificity control of the immunoprecipitation was carried out with an irrelevant anti-HA antibody (E). The results are representative of at least three independent experiments. Download figure Download PowerPoint Loss of p62 impairs allergic airway disease Since Th2 cells are important for allergic airway inflammation in a model of OVA-induced allergic airway disease, and p62 is required for optimal Th2 polarization, mice either WT or p62−/− were sensitized to OVA and then challenged twice with aerosolized antigen or PBS on the same day. Forty-eight hours after the aerosol challenge, mice were killed and the lungs were examined histologically by hematoxylin–eosin (C) staining for eosinophilic infiltration and bronchoalveolar lavages (BAL) were performed to determine inflammatory cell recruitment. There was a robust increase in total BAL cell number in WT mice that were OVA-sensitized and challenged with aerosolized antigen as compared with PBS-challenged mice (Figure 6A, left panel), due especially to eosinophils (Figure 6A, right panel). However, these two parameters were reduced in p62−/− mice (Figure 6A), indicating that p62 is required for an optimal Th2-dependent inflammatory response. Consistent with this notion, HE histological analysis of lung sections from this experiment shows that whereas the challenged WT mice display major inflammation with massive perivascular and peribronchial infiltration with abundant eosinophils, p62−/− mice show a much more attenuated response (Figure 6B). In addition, IL-13, IL-5 and eotaxin levels in BAL (Figure 6C), which were dramatically increased in OVA-challenged WT mice, were severely reduced in similarly treated p62−/− mice. Collectively, these results demonstrate that p62 is necessary for asthma in vivo, and suggest that p62 is required for optimal Th2 differentiation and function. Figure 6.Role of p62 in OVA-induced allergic airway disease. Allergic airway inflammation was OVA induced in WT and p62−/− (KO), and total cell numbers and eosinophils in BAL fluids were determined (A). Lung sections were prepared and stained with H&E (B). Levels of IL-13, IL-5 and eotaxin were determined in BAL fluids by ELISA (C). Data shown are representative of two experiments that each time involved four mice of each genotype (B). Data in panels A and C are the mean±s.d. of each genotype by duplicate. Download figure Download PowerPoint Discussion Although the presence of IL-12 or IL-4 in the culture media of differentiating T lymphocytes is important for the polarization of these cells along the Th1 or the Th2 lineages, respectively, it is also clear that signals emanating from the TCR are critical for the commitment of these cells along each polarization program (Murphy and Reiner, 2002). Recently, several signaling molecules have been shown to play important roles during Th2 differentiation. These include, for example, SLAM, SAP and SLAT (Fields and Flavell, 2001; Wu et al, 2001; Tanaka et al, 2003; Cannons et al, 2004). Experiments in which SLAT was ectopicall
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