Mapping Immune Reactivity toward Rv2653 and Rv2654: Two Novel Low‐Molecular‐Mass Antigens Found Specifically in the Mycobacterium tuberculosis Complex
2004; Oxford University Press; Volume: 189; Issue: 5 Linguagem: Inglês
10.1086/381679
ISSN1537-6613
AutoresClaus Aagaard, Inger Brock, Anja Weinreich Olsen, Tom H. M. Ottenhoff, Karin Weldingh, Peter Andersen,
Tópico(s)Mycobacterium research and diagnosis
ResumoNew tools are urgently needed for the detection of latent tuberculosis (TB). We evaluated the diagnostic potential of 2 novel Mycobacterium tuberculosis complex-specific candidate antigens (Rv2653 and Rv2654) and investigated T cell recognition during natural infection in humans and experimental infection in guinea pigs. Peripheral blood mononuclear cells stimulated with peptide pools covering the full length of Rv2654 induced interferon- gamma release in 10 of 19 patients with TB. Neither Rv2654 single peptides nor Rv2654 pools were recognized by bacille Calmette-Guerin-vaccinated donors. However, peptides from Rv2653 were recognized by both patients group. The cross-reactive epitope(s) in Rv2653 were located in a 36-amino acid stretch in the center of the molecule. Rv2654 also induced M. tuberculosis-specific skin-test responses in 3 of 4 aerosol-infected guinea pigs. Rv2654 is a strongly recognized T cell antigen that is highly specific for TB and has potential as a novel cell-mediated immunity-based TB diagnostic agent.
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