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Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence

2013; Wiley; Volume: 52; Issue: 23 Linguagem: Inglês

10.1002/anie.201301490

1521-3773

Samuel C. Reddington, P.J. Rizkallah, Peter Watson, Rachel Pearson, Eric M. Tippmann, D. Dafydd Jones,

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Angewandte Chemie International EditionVolume 52, Issue 23 p. 5974-5977 Communication Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence† Samuel C. Reddington, Samuel C. Reddington School of Biosciences, Cardiff University, Cardiff (UK) School of Chemistry, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Pierre J. Rizkallah, Dr. Pierre J. Rizkallah School of Medicine, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Peter D. Watson, Dr. Peter D. Watson School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorRachel Pearson, Rachel Pearson School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Eric M. Tippmann, Corresponding Author Dr. Eric M. Tippmann [email protected] School of Chemistry, Cardiff University, Cardiff (UK) Current address: Department of Chemistry, Indiana–Purdue University Fort Wayne, Fort Wayne, IN 46815 (USA) Eric M. Tippmann, School of Chemistry, Cardiff University, Cardiff (UK) D. Dafydd Jones, School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. D. Dafydd Jones, Corresponding Author Dr. D. Dafydd Jones [email protected] School of Biosciences, Cardiff University, Cardiff (UK) Eric M. Tippmann, School of Chemistry, Cardiff University, Cardiff (UK) D. Dafydd Jones, School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this author Samuel C. Reddington, Samuel C. Reddington School of Biosciences, Cardiff University, Cardiff (UK) School of Chemistry, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Pierre J. Rizkallah, Dr. Pierre J. Rizkallah School of Medicine, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Peter D. Watson, Dr. Peter D. Watson School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorRachel Pearson, Rachel Pearson School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. Eric M. Tippmann, Corresponding Author Dr. Eric M. Tippmann [email protected] School of Chemistry, Cardiff University, Cardiff (UK) Current address: Department of Chemistry, Indiana–Purdue University Fort Wayne, Fort Wayne, IN 46815 (USA) Eric M. Tippmann, School of Chemistry, Cardiff University, Cardiff (UK) D. Dafydd Jones, School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this authorDr. D. Dafydd Jones, Corresponding Author Dr. D. Dafydd Jones [email protected] School of Biosciences, Cardiff University, Cardiff (UK) Eric M. Tippmann, School of Chemistry, Cardiff University, Cardiff (UK) D. Dafydd Jones, School of Biosciences, Cardiff University, Cardiff (UK)Search for more papers by this author First published: 25 April 2013 https://doi.org/10.1002/anie.201301490Citations: 38 † The authors would like to thank the BBSRC (BB/H003746/1 and BB/E007384), SARTRE (G0900868), and EPSRC (EP/J015318/1 and EP/H045848/1) for funding. S.C.R. was supported by a MRC studentship supplemented by School of Chemistry. The authors thank Prof. Barry Carpenter and Dr. Simon Pope for useful discussions concerning fluorescence modulation mechanisms, Dr. Ian Brewis and the CBS Proteomics Facility for mass spectrometry, DLS for access to the synchrotron facility, Dr. David Cole and Anna Fuller for assisting with crystallography, and Johanna Jones and Rebecca Thompson for help with preliminary experiments. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description anie_201301490_sm_miscellaneous_information.pdf679.9 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume52, Issue23June 3, 2013Pages 5974-5977 RelatedInformation