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Non-invasive fetal RHD and RHCE genotyping from maternal plasma in alloimmunized pregnancies

2005; Wiley; Volume: 25; Issue: 12 Linguagem: Inglês

10.1002/pd.1282

1097-0223

Ilona Hromadníková, Kateřina Veselá, Blanka Benesova, K. Nekovarova, Daniela Dušková, R. Vlk, I. Špálová, Romana Gerychová, A. Hakenova, Z. Rosenbaumova, Pavel Vlašín, Alena Vlachova, V. Palasek, E. Roznakova, Pavel Calda,

Prenatal Screening and Diagnostics

In this prospective study, we assessed the feasibility of fetal RH genotyping by analysis of DNA extracted from maternal plasma samples of alloimmunized pregnant women using real-time PCR and primers and probes targeted toward RHD (exon 7 and exon 10) and RHCE (intron 2 and exon 5) genes.We analysed 23 alloimmunized pregnant women (16 anti-D, 5 anti-D + C, 2 anti-E) at risk of haemolytic disease of the newborn (HDN) within 11th and 37th week of pregnancy and correlated the results with serological analysis of cord blood.Detection of the presence of the RHD gene, the C and/or E alleles of the RHCE gene in maternal plasma samples is highly accurate and enables implementation in a clinical diagnostic algorithm for following pregnancies at risk for HDN. The absence of RHD gene, the C and/or E alleles of RHCE gene in the current pregnancy excludes the risk of HDN caused by anti-D, anti-C and/or anti-E alloantibodies and the performance of invasive fetal-blood sampling.