The adenovirus E4-ORF4 splicing enhancer protein interacts with a subset of phosphorylated SR proteins
2001; Springer Nature; Volume: 20; Issue: 4 Linguagem: Inglês
10.1093/emboj/20.4.864
ISSN1460-2075
AutoresCamilla Nilsson, Svend K. Petersen‐Mahrt, Céline Durot, Ronit Shtrichman, Adrian R. Krainer, Tamar Kleinberger, Göran Akusjärvi,
Tópico(s)RNA Research and Splicing
ResumoArticle15 February 2001free access The adenovirus E4-ORF4 splicing enhancer protein interacts with a subset of phosphorylated SR proteins Camilla Estmer Nilsson Camilla Estmer Nilsson Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Search for more papers by this author Svend Petersen-Mahrt Svend Petersen-Mahrt Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Present address: MRC LMB, Hills Road, Cambridge, CB2 2QH UK Search for more papers by this author Céline Durot Céline Durot ENS Lyon, 69364 Lyon, Cedex, France Search for more papers by this author Ronit Shtrichman Ronit Shtrichman The Gonda Center of Molecular Microbiology, The B.Rappaport Faculty of Medicine, Technion, Haifa, 31096 Israel Present address: MCDB Department, University of California, Santa Barbara, CA, 93106 USA Search for more papers by this author Adrian R. Krainer Adrian R. Krainer Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724 USA Search for more papers by this author Tamar Kleinberger Tamar Kleinberger The Gonda Center of Molecular Microbiology, The B.Rappaport Faculty of Medicine, Technion, Haifa, 31096 Israel Search for more papers by this author Göran Akusjärvi Corresponding Author Göran Akusjärvi Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Search for more papers by this author Camilla Estmer Nilsson Camilla Estmer Nilsson Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Search for more papers by this author Svend Petersen-Mahrt Svend Petersen-Mahrt Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Present address: MRC LMB, Hills Road, Cambridge, CB2 2QH UK Search for more papers by this author Céline Durot Céline Durot ENS Lyon, 69364 Lyon, Cedex, France Search for more papers by this author Ronit Shtrichman Ronit Shtrichman The Gonda Center of Molecular Microbiology, The B.Rappaport Faculty of Medicine, Technion, Haifa, 31096 Israel Present address: MCDB Department, University of California, Santa Barbara, CA, 93106 USA Search for more papers by this author Adrian R. Krainer Adrian R. Krainer Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724 USA Search for more papers by this author Tamar Kleinberger Tamar Kleinberger The Gonda Center of Molecular Microbiology, The B.Rappaport Faculty of Medicine, Technion, Haifa, 31096 Israel Search for more papers by this author Göran Akusjärvi Corresponding Author Göran Akusjärvi Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden Search for more papers by this author Author Information Camilla Estmer Nilsson1, Svend Petersen-Mahrt1,2, Céline Durot3, Ronit Shtrichman4,5, Adrian R. Krainer6, Tamar Kleinberger4 and Göran Akusjärvi 1 1Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden 2Present address: MRC LMB, Hills Road, Cambridge, CB2 2QH UK 3ENS Lyon, 69364 Lyon, Cedex, France 4The Gonda Center of Molecular Microbiology, The B.Rappaport Faculty of Medicine, Technion, Haifa, 31096 Israel 5Present address: MCDB Department, University of California, Santa Barbara, CA, 93106 USA 6Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2001)20:864-871https://doi.org/10.1093/emboj/20.4.864 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info SR proteins purified from uninfected HeLa cells inhibit adenovirus IIIa pre-mRNA splicing by binding to the intronic IIIa repressor element (3RE). In contrast, SR proteins purified from late adenovirus-infected cells are functionally inactivated as splicing repressor proteins by a virus-induced dephosphorylation. We have shown that the adenovirus E4-ORF4 protein, which binds the cellular protein phos phatase 2A (PP2A) and activates IIIa splicing in vitro and in vivo , induces SR protein dephosphorylation. Here we show that E4-ORF4 interacts with only a subset of SR proteins present in HeLa cells. Thus, E4-ORF4 interacts efficiently with SF2/ASF and SRp30c, but not with other SR proteins. Interestingly, E4-ORF4 interacts with SF2/ASF through the latter's RNA recognition motifs. Furthermore, E4-ORF4 interacts preferentially with the hyperphosphorylated form of SR proteins found in uninfected HeLa cells. E4-ORF4 mutant proteins that fail to bind strongly to PP2A or SF2/ASF do not relieve the repressive effect of HeLa SR proteins on IIIa pre-mRNA splicing in transient transfection experiments, suggesting that an interaction between all three proteins is required for E4-ORF4-induced SR protein dephosphorylation. Introduction The adenovirus E4-ORF4 protein is a 14 kDa protein that binds to the B55 and B56 subunits of protein phosphatase 2A (PP2A) (Kleinberger and Shenk, 1993; Shtrichman et al., 2000). PP2A is a serine/threonine phosphatase consisting of a core dimer of the A subunit and the catalytic C subunit, which interacts with a family of regulatory B subunits (Kamibayashi et al., 1994; McCright and Virshup, 1995; Csortos et al., 1996). A wealth of data implicate PP2A in the regulation of a number of cellular functions, but the mechanisms by which B subunits target the core dimer to different substrates are not well understood (reviewed in Wera and Hemmings, 1995; Millward et al., 1999). The E4-ORF4–PP2A complex reduces E1A activation of AP-1 transcription factor activity (Muller et al., 1992). This reduction in transcription factor activity correlates with an increased accumulation of underphosphorylated forms of E1A and c-Fos. E4-ORF4 also inhibits E1A activation of junB (Kleinberger and Shenk, 1993), and adenovirus E2 (Mannervik et al., 1999) and E4 transcription (Bondesson et al., 1996). E4-ORF4 has also been shown to induce p53-independent apoptosis (Lavoie et al., 1998; Marcellus et al., 1998; Shtrichman and Kleinberger, 1998) by a mechanism requiring E4-ORF4 binding to PP2A (Marcellus et al., 2000; Shtrichman et al., 2000). We have shown further that E4-ORF4 regulates adenovirus L1 splicing, and induces SR protein dephosphorylation (Kanopka et al., 1998). Collectively, available evidence suggests that E4-ORF4 regulates transcription, splicing and apoptosis through its binding to PP2A. Potentially the effect of E4-ORF4 on apoptosis is the result of its regulatory role on transcription factor and/or splicing factor activity. The adenovirus L1 unit is an example of a gene whose last intron is spliced using a common 5′ splice site and two competing 3′ splice sites, generating two predominant cytoplasmic L1 mRNAs: the 52,55K (proximal 3′ splice site) or the IIIa (distal 3′ splice site) mRNAs, respectively (reviewed in Imperiale et al., 1995). L1 splicing is regulated during the infectious cycle. Thus, the 52,55K mRNA is the only L1 mRNA produced early after infection, whereas the IIIa mRNA becomes the predominant L1 mRNA accumulating late after infection (Chow et al., 1979; Akusjärvi and Persson, 1981; Nevins and Wilson, 1981; Svensson and Akusjärvi, 1986). We have shown previously that SR proteins purified from HeLa cells (SR-HeLa) inhibit IIIa splicing by binding to a purine-rich intronic repressor element, the 3RE, located immediately upstream of the IIIa branch site (Kanopka et al., 1996). SR protein binding to the 3RE blocks splicing by inhibiting U2 snRNP recruitment to the IIIa branch site. SR proteins are a family of essential splicing factors required for constitutive splicing and functioning as regulators of alternative splicing (reviewed in Fu, 1995; Manley and Tacke, 1996; Graveley, 2000). SR proteins have one or two N-terminal RNA recognition motifs (RRMs) and a C-terminus rich in arginine/serine dipeptide repeats (the RS domain), which mediates protein interactions with other components of the splicing machinery (Zahler et al., 1992; Caceres and Krainer, 1993; Wu and Maniatis, 1993; Zuo and Manley, 1993; Kohtz et al., 1994). SR proteins are highly phosphorylated in vivo (Roth et al., 1990), mainly in the RS domain (Colwill et al., 1996), a modification that is required for their function in spliceosome assembly (Mermoud et al., 1994; Roscigno and Garcia-Blanco, 1995; Xiao and Manley, 1997). Thus, phosphorylated SR proteins are needed for initiation of spliceosome assembly, and SR protein dephosphorylation appears to be required for splicing catalysis (Cao et al., 1997). On the other hand, the level of SR protein phosphorylation appears to be critical, since both hyperphosphorylation and hypophosphorylation inhibit their activity as activators of splicing (Kanopka et al., 1998; Prasad et al., 1999), in both constitutive and activated alternative splicing. The phosphorylated status of SR proteins has also been shown to control their localization and transport in the nucleus between speckles and sites of active transcription and splicing. Thus, phosphorylation appears to release SR proteins from their storage sites and target them to sites of transcription and splicing in the nucleus (Colwill et al., 1996; Caceres et al., 1998; Misteli et al., 1998). We have shown previously that, during an adenovirus infection, SR proteins become functionally inactivated as IIIa splicing repressor proteins by a virus-induced dephosphorylation (Kanopka et al., 1998). This post-translational modification reduces their capacity to bind to the 3RE and, as a consequence, alleviates their repressive effect on IIIa splicing. We further showed that the virus-encoded E4-ORF4 protein induces SR protein dephosphorylation, and has the capacity to activate IIIa pre-mRNA splicing, both in vitro and in transiently transfected cells (Kanopka et al., 1998). Here we determine the binding specificity of E4-ORF4 interaction with SR proteins. Much to our surprise, we find that E4-ORF4 interacts specifically with only a subset of the SR proteins expressed in HeLa cells. Thus, E4-ORF4 interacts efficiently with SF2/ASF (also named ASF/SF2) and SRp30c, but not with SRp20, SC35, SRp40, SRp55 or SRp75. The specificity appears to result from the fact that E4-ORF4 associates with SF2/ASF through the latter's RRMs. Furthermore, E4-ORF4 selectively interacts with the hyperphosphorylated form of SR proteins. Previously we showed that the 3RE is necessary for E4-ORF4 activation of IIIa splicing (Kanopka et al., 1998). Here we extend this finding by demonstrating that the 3RE is sufficient for E4-ORF4-mediated activation of splicing. Moreover, we show that E4-ORF4 mutant proteins that show reduced binding to SF2/ASF or inefficient recruitment of an active PP2A phosphatase complex do not activate IIIa splicing in transient transfection experiments. Results E4-ORF4 interacts selectively with SF2/ASF and SRp30c To study the specificity of E4-ORF4 interaction with SR proteins, we incubated GST–E4-ORF4 with 35S-labeled SR proteins produced by coupled in vitro transcription–translation. As shown in Figure 1, glutathione S-transferase (GST)–E4-ORF4 interacts efficiently with SF2/ASF and SRp30c, but only weakly, if at all, with SRp20, SC35, SRp40, SRp55 or SRp75 in a GST pull-down assay. Interestingly, the RS domain of SF2/ASF is not important for this interaction, since SF2-ΔRS interacts at least as efficiently as wild-type SF2/ASF with E4-ORF4 (Figure 2B, lanes 1–6). In contrast, deletion of RRM1 of SF2/ASF abolished binding (Figure 2B, lanes 7–9). Figure 1.E4-ORF4 interacts selectively with SF2/ASF and SRp30c. GST or GST–E4-ORF4 was incubated with the indicated 35S-labeled SR proteins. Bound proteins were separated by gel electrophoresis and visualized by autoradiography. Download figure Download PowerPoint Figure 2.E4-ORF4 interacts with SF2/ASF through its RNA recognition motifs. (A) Schematic illustration showing the structure of SF2/ASF mutant proteins used in (B) and (C). (B) An in vitro binding assay using GST–E4-ORF4 and 35S-labeled SF2/ASF wild-type and mutant proteins. In lanes 13–15, the in vitro transcribed/translated 35S-labeled SF2/ASF extract was treated with 5 μg of RNase A for 15 min at 37°C before incubation with GST–E4-ORF4. (C) Extracts prepared from 293 cells co-transfected with pCMV-E4-ORF4 and plasmids encoding T7-tagged wild-type or mutant SF2/ASF proteins or SC35 were immunoprecipitated with an anti-E4-ORF4 antibody. Proteins were separated by gel electrophoresis and transferred to a membrane. SF2/ASF and SC35 proteins were detected using an anti-T7 antibody in a western blot assay. In lanes 11 and 12, the cell extract was treated with RNase A before immunoprecipitation with E4-ORF4. Download figure Download PowerPoint The requirement for the RRMs of SF2/ASF for E4-ORF4 interaction was confirmed further in vivo. For this experiment, 293 cells were co-transfected with plasmids encoding wild-type E4-ORF4 and T7-tagged SF2/ASF mutant proteins. As shown in Figure 2C, immunoprecipitation with an anti-E4-ORF4 antibody co-immunoprecipitated wild-type SF2/ASF and SF2-ΔRS, but not SF2-RRM1/RS or SF2-RRM2/RS, confirming the in vitro binding data (Figure 2B). Furthermore, E4-ORF4 did not interact with SC35 in vivo (Figure 2C, lanes 13 and 14), reinforcing the conclusion from the in vitro binding studies (Figure 1) suggesting that E4-ORF4 interaction with SF2/ASF is specific. Probing the western blot with anti-E4-ORF4 demonstrated that E4-ORF4 was expressed similarly in all transfections (data not shown). The observation that the RRMs of SF2/ASF are required for interaction with E4-ORF4 could indicate that E4-ORF4 becomes tethered indirectly to SF2/ASF through simultaneous, but independent, interaction with contaminating RNA in the extract. However, RNase treatment of the in vitro translated 35S-labeled SF2/ASF did not disrupt the interaction with E4-ORF4 in vitro (Figure 2B, lanes 13–15) or in vivo (Figure 2C, lanes 11–12), suggesting that the SF2/ASF interaction with E4-ORF4 is direct. Also, the observation that SRp20, SC35, SRp40, SRp55 and SRp75, which would also be expected to bind RNA in the TNT extract, did not interact with E4-ORF4, further supports our conclusion that RNA does not unspecifically mediate the E4-ORF4 interaction with SF2/ASF. Collectively, our results show that E4-ORF4 interacts efficiently with a subset of HeLa SR proteins: SF2/ASF and SRp30c. Moreover, E4-ORF4 appears to associate specifically with SF2/ASF through its RRMs. E4-ORF4 interacts with hyperphosphorylated SR proteins SR proteins are highly phosphorylated in vivo, a modification that is important for their function in spliceosome assembly and splicing catalysis (reviewed in Misteli, 1999). Since E4-ORF4 induces SR protein dephosphorylation, E4-ORF4 might be expected to interact preferentially with hyperphosphorylated SR proteins. To test this hypothesis, we compared the capacity of E4-ORF4 to interact with SR-HeLa and SR proteins purified from late adenovirus-infected cells (SR-Ad). For this experiment, equal amounts of SR-HeLa and SR-Ad were separated by gel electrophoresis and transferred to a membrane. As shown in Figure 3A, probing the filter with the phospho-epitope-specific monoclonal antibody 104 (mAb104; Zahler et al., 1992) demonstrated that SR-HeLa proteins were recognized efficiently by mAb104, whereas SR-Ad were not (lanes 1 and 4). Interestingly, incubating SR-Ad with a small amount of HeLa cell nuclear extract (HeLa-NE) restored the phospho-epitope(s) recognized by mAb104 to SR-Ad (lane 5), whereas incubation in HeLa-NE pre-treated with hexokinase and glucose to deplete the small ATP pool present in a dialyzed extract did not restore the phospho-epitope(s) (lane 6). The small amount of HeLa-NE used in this phosphorylation assay did not contribute detectable amounts of SR proteins (data not shown). Note that the proteins recognized by mAb104 in SR-Ad migrate slightly faster than SR-HeLa (compare the SRp30 fraction in lanes 1 and 4), a result that is compatible with the conclusion that the SR proteins recognizable by mAb104 in SR-Ad are hypophosphorylated. Also, incubating SR-Ad in HeLa-NE resulted in a reduction in mobility such that SR-Ad migration was identical to that of SR-HeLa (lane 5). Taken together, these results support our previous conclusion that SR-Ad proteins are hypophosphorylated (Kanopka et al., 1998), and furthermore suggest that SR-Ad can be reconverted to SR proteins with the same electrophoretic mobility and mAb104-recognizable phospho-epitope(s) as SR-HeLa by in vitro phosphorylation. Figure 3.E4-ORF4 interacts preferentially with hyperphosphorylated SR proteins. (A) SR proteins prepared from HeLa cells (SR-HeLa, lane 1) and adenovirus-infected HeLa cells (SR-Ad, lane 4) were pre-incubated in nuclear extract (HeLa-NE) or nuclear extract depleted of ATP [HeLa-NE(–ATP)] (lanes 2, 3, 5 and 6). Samples were separated on a 10% SDS–polyacrylamide gel, transferred to a membrane and probed with mAb104 (an SR protein-specific antibody that detects phospho-epitopes in the RS domain) in a western blot assay. (B) The filter was probed with mAb96 (an SF2/ASF-specific antibody that detects the RRM1 of SF2/ASF) in a western blot assay. (C) The filter was probed with GST–E4-ORF4 in a far western blot assay. E4-ORF4-interacting proteins were visualized by chemiluminescence using an anti-E4-ORF4 antibody. Note that the autoradiograms shown are taken from different experiments. Download figure Download PowerPoint To measure total SF2/ASF levels, we reprobed the filter with mAb96 (Hanamura et al., 1998), which recognizes RRM1 of SF2/ASF. As shown in Figure 3B, two distinct populations of SF2/ASF, with clear differences in migration, can be detected in SR-HeLa and SR-Ad, respectively (compare lanes 1 and 4). The slower migrating species is recognized by mAb104. Treatment of SR-HeLa with calf intestinal alkaline phosphatase resulted in an accumulation of the faster migrating species observed in SR-Ad (data not shown). We conclude that the two species represent hyperphosphorylated and unphosphorylated forms of SF2/ASF. Interestingly, the weak mAb104 signal seen in the SRp30 fraction in SR-Ad (Figure 3A, lane 4) migrates at a position between the highly and unphosphorylated SF2ASF, a result strengthening the conclusion that SR-Ad is hypophosphorylated (Kanopka et al., 1998). The results extend our previous results by suggesting that most of SF2/ASF is unphosphorylated in late adenovirus-infected cells. Furthermore, the results show that incubation of SR-Ad in HeLa-NE produces a quantitative shift in mobility of SF2/ASF to the slower migrating species seen in SR-HeLa (compare lanes 4 and 5). Interestingly, reprobing the filter with GST–E4-ORF4 in a far western blot assay showed that E4-ORF4 interacts efficiently only with the SRp30 fraction in SR-HeLa but not in SR-Ad (Figure 3C, lanes 1 and 4). Importantly, in vitro phosphorylation of SR-Ad restored E4-ORF4 interaction to the slower, mAb104-recognizable species (Figure 3C, lane 5). Collectively, these results suggest that E4-ORF4 interacts preferentially with hyperphosphorylated SR proteins, and support our binding studies (Figure 1) by indicating that E4-ORF4 interacts selectively with the SRp30 fraction, which contains SF2/ASF and probably SRp30c. It is noteworthy that E4-ORF4 also interacts with a cluster of slower migrating proteins, in a phosphorylation-dependent manner (Figure 3C). However, these interactions are only seen in reactions containing HeLa-NE (Figure 3C, lanes 2, 3, 5 and 6), suggesting that they represent E4-ORF4 interactions with other host cell proteins. Coomassie Blue staining of identical gels showed that equal amounts of SR proteins were analyzed in each sample (data not shown). The 3RE is necessary and sufficient for E4-ORF4 activation of splicing We have shown previously that the 3RE is necessary for E4-ORF4 activation of IIIa pre-mRNA splicing in a transient transfection assay (Kanopka et al., 1998). Thus, replacing the 49 nucleotide 3RE with the corresponding sequence from the rabbit β-globin first intron abolished E4-ORF4 stimulation of IIIa splicing. More recently we have shown that IIIa splicing is also regulated by a second viral element, the IIIa virus infection-dependent splicing enhancer (the 3VDE; Mühlemann et al., 2000). This finding prompted us to re-examine whether the 3RE was the only element required for E4-ORF4 activation of splicing. For this experiment, we reconstructed eukaryotic expression vectors for some of the IIIa–β-globin hybrid constructs previously used in our in vitro studies (Kanopka et al., 1996). As shown in Figure 4, transfer of the 3RE to β-globin significantly reduced β-globin splicing [transcript glob (3RE), compare lanes 1 and 3]. This finding is in agreement with our in vitro experiments showing that the 3RE is the minimal sequence element required for SR protein inhibition of splicing (Kanopka et al., 1996). Importantly, co-transfection of a plasmid encoding E4-ORF4 stimulated β-globin splicing and annulled the repressive effect of the 3RE (compare lanes 1 and 4). In agreement with our previous demonstration that the 3VDE only functions efficiently in the context of virus-infected cells (Mühlemann et al., 2000), transfer of the 3VDE to β-globin resulted in a severe inhibition of β-globin splicing [transcript glob (3VDE), lanes 5]. However, compared with glob (3RE) splicing, glob (3VDE) splicing was only marginally activated by E4-ORF4 co-transfection (lane 6). Collectively, our data show that the 3RE is the primary element conferring an E4-ORF4-enhanced splicing phenotype, and show furthermore that the 3RE is both necessary (Kanopka et al., 1998) and sufficient for E4-ORF4 activation of splicing (Figure 4). Figure 4.The 3RE is sufficient to confer an E4-ORF4-enhanced splicing phenotype to β-globin. (A) Schematic representation of the IIIa–β-globin hybrid reporter transcripts used. (B) Quantitative S1 analysis of IIIa–β-globin hybrid mRNA levels in HeLa cells co-transfected with pCMV-E4-ORF4 and the reporter plasmids depicted in (A). Average splicing efficiencies and standard deviations are shown (three experiments). Download figure Download PowerPoint Strong E4-ORF4 interaction with SF2/ASF and efficient E4-ORF4 recruitment of a functional phosphatase complex correlate with activation of IIIa pre-mRNA splicing Our working hypothesis predicts that E4-ORF4 functions as a specificity factor that targets the SR protein SF2/ASF for PP2A dephosphorylation. To test this model, we analyzed the capacity of E4-ORF4 mutant proteins to interact with SF2/ASF in vitro and to activate IIIa pre-mRNA splicing in a transient transfection assay. In a recent study, Shtrichman et al. (1999) established the capacity of E4-ORF4 mutant proteins to bind PP2A and co-immunoprecipitate an active phosphatase complex. Here we used this collection of E4-ORF4 mutant plasmids (Figure 5A) to study E4-ORF4 interaction with SF2/ASF in vitro (Figure 5B) and, furthermore, their capacity to activate IIIa pre-mRNA splicing in transiently transfected HeLa cells (Figure 5C). Care was taken to ensure that equal amounts of E4-ORF4 mutant proteins were used in the assays (data not shown). Figure 5.Capacity of E4-ORF4 mutant proteins to bind to SF2/ASF and activate IIIa pre-mRNA splicing. (A) Schematic representation of E4-ORF4 showing the position of mutated sequences in the E4-ORF4 mutant proteins. The interactions are summarized at the top. Gray boxes indicate regions essential for strong interaction of E4-ORF4 with SF2/ASF [see (B)], and efficient E4-ORF4 recruitment of a functional PP2A phosphatase complex (Shtrichman et al., 1999). Thin lines indicate regions significantly affecting SF2/ASF binding and phosphatase recruitment. (B) In vitro binding assay showing the interaction capacity of 35S-labeled SF2/ASF and GST–E4-ORF4 mutant proteins. (C) E4-ORF4 activation of IIIa pre-mRNA splicing. Quantitative S1 nuclease analysis of IIIa mRNA levels in HeLa cells co-transfected with pCMV plasmids encoding E4-ORF4 mutant proteins and the reporter pAdCMV-IIIa (schematically shown at the top). Average splicing efficiencies and standard deviations are shown (three experiments). Download figure Download PowerPoint As shown in Figure 5B, wild-type E4-ORF4, and mutants R2, A11, R3-9 and A10 interact efficiently with 35S-labeled SF2/ASF in a GST pull-down assay. E4-ORF4 mutants p20, R4 and R3-2 show reduced binding affinity, whereas mutants R1, 12 and A3 lost almost all of the binding capacity. This analysis suggests that E4-ORF4 sequences critical for SF2/ASF binding map to three non-overlapping E4-ORF4 sequence elements (Figure 5A). Thus, the N-terminus and regions located around amino acids 61 and 95 are essential for E4-ORF4–SF2/ASF interaction. The lack of a simple primary amino acid sequence determinant of binding indicates that the E4-ORF4–SF2/ASF interaction may depend on E4-ORF4 protein conformation. Strong E4-ORF4 interaction with SF2/ASF is not sufficient for activated IIIa splicing. Thus, mutants R2, A11, R3-9 and A10 interact efficiently with SF2/ASF (Figure 5B), whereas only mutant A11 enhances IIIa splicing (Figure 5C). However, comparing the binding and splicing activation results with the capacity of the E4-ORF4 mutant proteins to interact functionally with PP2A (Shtrichman et al., 1999) shows that strong interaction of E4-ORF4 with both SF2/ASF and PP2A correlates with IIIa splicing activation. Thus, mutants that show a strong interaction with one component were weak activators of IIIa splicing (Figure 5C; mutants R2, 12, R4 and A10), whereas mutants that did not either interact efficiently with SF2/ASF or recruit an active phosphatase (Figure 5C; mutants R1, p20, R3-2 and A3) slightly repressed IIIa splicing. In contrast, mutant A11, which shows strong interaction with SF2/ASF and is efficient in recruiting an active phosphatase, was almost as efficient as the wild-type E4-ORF4 protein in activating IIIa splicing. Mutant R3-9 is the only E4-ORF4 protein that does not obey this general rule (see Discussion). Discussion We have shown previously that E4-ORF4 activates IIIa pre-mRNA splicing both in vivo and in vitro and, furthermore, induces SR protein dephosphorylation (Kanopka et al., 1998). The results presented here reveal several novel aspects of the mechanisms by which E4-ORF4 regulates SR protein activity and RNA splicing. (i) The SR protein-responsive 3RE is sufficient to confer an E4-ORF4-activated splicing phenotype to a heterologous pre-mRNA (Figure 4). (ii) E4-ORF4 interacts selectively with a subset of HeLa SR proteins, namely SF2/ASF and SRp30c (Figure 1). (iii) E4-ORF4 interacts preferentially with the hyperphosphorylated form of SR proteins (Figure 3). (iv) E4-ORF4 activation of IIIa pre-mRNA splicing correlates with strong interaction of E4-ORF4 with an active PP2A and SF2/ASF (Figures 5 and 6). Figure 6.Strong E4-ORF4 interaction with SF2/ASF, and E4-ORF4 recruitment of a functional phosphatase complex correlate with E4-ORF4 activation of IIIa pre-mRNA splicing. The figure presents a summary of E4-ORF4 mutant protein interactions with SF2/ASF (Figure 5B), E4-ORF4 recruitment of a functional phosphatase complex (Shtrichman et al., 1999) and the capacity of E4-ORF4 mutant proteins to activate IIIa pre-mRNA splicing in a transient transfection assay (Figure 5C). *The percentage binding or recruitment are as follows: −, 0–30%; (+), 30–60%; and +, 60–100%. Download figure Download PowerPoint The E4-ORF4 mutant analysis shows an almost perfect correlation between strong E4-ORF4 interaction with SF2/ASF, E4-ORF4 recruitment of an active phosphatase complex and IIIa splicing activation (Figure 6). Thus, strong interaction between E4-ORF4 and only one component is not enough to activate IIIa splicing in a transient transfection assay. For example, mutant 12 recruits an active phosphatase complex but does not interact efficiently with SF2/ASF, and is defective in IIIa pre-mRNA splicing activation. Mutant R2 shows the opposite binding specificity, i.e. it binds SF2/ASF but does not recruit an active phosphatase, and it is similarly defective in activation of splicing. Moreover, mutants with reduced binding affinity for SF2/ASF or reduced phosphatase recruitment do not stimulate IIIa pre-mRNA splicing, whereas mutant A11, which interacts efficiently with both SF2/ASF and PP2A, is a strong activator of IIIa pre-mRNA splicing. The only E4-ORF4 mutant protein that does not conform to this general rule is mutant R3-9, which does not activate IIIa splicing despite the fact that it interacts efficiently with SF2/ASF in vitro and recruits an active phosphatase complex (Figure 6). We do not know the significance of this observation. However, we note that E4-ORF4 binding to PP2A and E4-ORF4 recruitment of an active phosphatase complex similarly did not show a perfect match (Shtrichman et al., 1999). Thus, E4-ORF4 binding to PP2A is not synonymous with active dephosphorylation. The simplest explanation for this discrepancy between binding and dephosphorylation is that the E4-ORF4–PP2A interaction requires a higher order structure that correctly presents the target protein to the PP2A catalytic subunit. Thus, mutant R3-9 interaction with SF2/ASF and PP2A may be non-productive and fail to present the SR protein correctly for catalysis. However, we cannot exclude the possibility that E4-ORF4 targets another important
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