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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

2014; Dove Medical Press; Linguagem: Inglês

10.2147/ijn.s53432

1178-2013

S Pappalardo, Cecilia Langellotti, Sebastián Di Giacomo, Valeria Olivera, Valeria Quattrocchi, Patricia Zamorano, William C. Hartner, Tatyana Levchenko, Vladimir P. Torchilin,

Immune Response and Inflammation

Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.