Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Detection of Babesia bigemina in cattle of different genetic groups and in Rhipicephalus (Boophilus) microplus tick

2008; Elsevier BV; Volume: 155; Issue: 3-4 Linguagem: Inglês

10.1016/j.vetpar.2008.04.022

1873-2550

M. C. de S. Oliveira, T.C.G. Oliveira-Sequeira, Luciana Correia de Almeida Regitano, M. M. de Alencar, T. A. Néo, A. M. Silva, Henrique Nunes de Oliveira,

Vector-Borne Animal Diseases

Babesia bigemina infections were investigated in four genetic groups of beef cattle and in Rhipicephalus (Boophilus) microplus engorged female ticks. Blood samples and engorged female ticks were collected from 15 cows and 15 calves from each of the following genetic groups: Nelore, Angus x Nelore, Canchim x Nelore, and Simmental x Nelore. Microscopic examination of blood smears and tick hemolymph revealed that merozoites of B. bigemina (6/60) as well as kinetes of Babesia spp. (9/549) were only detected in samples (blood and ticks, respectively) originated from calves. PCR-based methods using primers for specific detection of B. bigemina revealed 100% infection in both calves and cows, regardless the genetic group. Tick infection was detected by nested-PCR amplifications showing that the frequency of B. bigemina was higher (P 0.05).