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Light scattering of human red blood cells during metabolic remodeling of the membrane

2011; SPIE; Volume: 16; Issue: 1 Linguagem: Inglês

10.1117/1.3524509

1560-2281

YongKeun Park, Catherine Best‐Popescu, Ramachandra R. Dasari, Gabriel Popescu,

Spectroscopy Techniques in Biomedical and Chemical Research

We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.