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The Large GTPase Dynamin Associates with the Spindle Midzone and Is Required for Cytokinesis

2002; Elsevier BV; Volume: 12; Issue: 24 Linguagem: Inglês

10.1016/s0960-9822(02)01390-8

1879-0445

Heather M. Thompson, Ahna R. Skop, Ursula Euteneuer, Barbara J Meyer, Mark A. McNiven,

Photosynthetic Processes and Mechanisms

Cytokinesis involves the concerted efforts of the microtubule and actin cytoskeletons as well as vesicle trafficking and membrane remodeling to form the cleavage furrow and complete daughter cell separation (for reviews, see [1Glotzer M. Animal cell cytokinesis.Annu. Rev. Cell Dev. Biol. 2001; 17: 351-386Crossref PubMed Scopus (281) Google Scholar, 2Straight A.F. Field C.M. Microtubules, membranes and cytokinesis.Curr. Biol. 2000; 10: R760-R770Abstract Full Text Full Text PDF PubMed Scopus (151) Google Scholar, 3Robinson D.N. Spudich J.A. Towards a molecular understanding of cytokinesis.Trends Cell Biol. 2000; 10: 228-237Abstract Full Text Full Text PDF PubMed Scopus (150) Google Scholar, 4Bowerman B. Severson A.F. Cell division plant-like properties of animal cell cytokinesis.Curr. Biol. 1999; 9: R658-R660Abstract Full Text Full Text PDF PubMed Scopus (14) Google Scholar, 5O'Halloran T.J. Membrane traffic and cytokinesis.Traffic. 2000; 1: 921-926PubMed Google Scholar, 6Finger F.P. White J.G. Fusion and fission membrane trafficking in animal cytokinesis.Cell. 2002; 108: 727-730Abstract Full Text Full Text PDF PubMed Scopus (80) Google Scholar]). The exact mechanisms that support membrane remodeling during cytokinesis remain largely undefined. In this study, we report that the large GTPase dynamin, a protein involved in membrane tubulation and vesiculation [7McNiven M.A. Cao H. Pitts K.R. Yoon Y. The dynamin family of mechanoenzymes pinching in new places.Trends Biochem. Sci. 2000; 25: 115-120Abstract Full Text Full Text PDF PubMed Scopus (305) Google Scholar, 8Hinshaw J.E. Dynamin and its role in membrane fission.Annu. Rev. Cell Dev. Biol. 2000; 16: 483-519Crossref PubMed Scopus (565) Google Scholar], is essential for successful cytokinesis. Using biochemical and morphological methods, we demonstrate that dynamin localizes to the spindle midzone and the subsequent intercellular bridge in mammalian cells and is also enriched in spindle midbody extracts. In Caenorhabditis elegans, dynamin localized to newly formed cleavage furrow membranes and accumulated at the midbody of dividing embryos in a manner similar to dynamin localization in mammalian cells. Further, dynamin function appears necessary for cytokinesis, as C. elegans embryos from a dyn-1 ts strain [9Clark S.G. Shurland D.L. Meyerowitz E.M. Bargmann C.I. van der Bliek A.M. A dynamin GTPase mutation causes a rapid and reversible temperature-inducible locomotion defect in C. elegans.Proc. Natl. Acad. Sci. USA. 1997; 94: 10438-10443Crossref PubMed Scopus (97) Google Scholar], as well as dynamin RNAi-treated embryos, showed a marked defect in the late stages of cytokinesis. These findings indicate that, during mitosis, conventional dynamin is recruited to the spindle midzone and the subsequent intercellular bridge, where it plays an essential role in the final separation of dividing cells.