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Controlling Intracellular Macrocyclization for the Imaging of Protease Activity

2011; Wiley; Volume: 50; Issue: 10 Linguagem: Inglês

10.1002/anie.201006140

1521-3773

Deju Ye, Gaolin Liang, Man Lung, Jianghong Rao,

Photochromic and Fluorescence Chemistry

Reporters on the ground: An intramolecular macrocyclization reaction took place highly efficiently in live cells under the control of a specific enzyme and reduction by glutathione (GSH; see picture). Macrocyclic products (represented as blue rings) synthesized in cells self-assembled into nanoparticles aggregated and retained at the site near the enzyme location to report local proteolytic activity in live cells. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.