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Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

2010; Optica Publishing Group; Volume: 1; Issue: 2 Linguagem: Inglês

10.1364/boe.1.000687

2156-7085

Keisuke Isobe, Hiroshi Hashimoto, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa,

Photosynthetic Processes and Mechanisms

We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.