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Cytokine Production in Cell Culture by Peripheral Blood Mononuclear Cells from Immunocompetent Hosts

1998; American Society for Microbiology; Volume: 5; Issue: 1 Linguagem: Inglês

10.1128/cdli.5.1.78-81.1998

1098-6588

Rohit Katial, D M Sachanandani, C A Pinney, Michael M. Lieberman,

Viral Infectious Diseases and Gene Expression in Insects

ABSTRACT The production of interleukin 2 (IL-2) gamma interferon, IL-4, tumor necrosis factor alpha (TNF-α), TNF-β, IL-5, and IL-10 in vitro by peripheral blood mononuclear cells cultured from healthy immunocompetent subjects after mitogen stimulation was determined. The mitogens used were concanavalin A, phytohemagglutinin, pokeweed mitogen, and Staphylococcus aureus Cowen. The results obtained provide a normal range for the production of these cytokines under specified conditions in vitro.