Construction of Mobilizable Mini-Tn 7 Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy Promoter lux Reporter Analysis
2013; American Society for Microbiology; Volume: 79; Issue: 13 Linguagem: Inglês
10.1128/aem.00640-13
ISSN1098-5336
AutoresF. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, George W. Liechti, Herbert P. Schweizer, Joanna B. Goldberg,
Tópico(s)RNA and protein synthesis mechanisms
ResumoABSTRACT We describe the construction of mini-Tn 7 -based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn 7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
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