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Resolution of optical isomers of 2,3,4,6‐tetra‐ O ‐acetyl‐β‐D‐glucopyranosyl isothiocyanate (GITC)–derivatized DL‐amino acids by micellar electrokinetic chromatography

1990; Wiley; Volume: 2; Issue: 5 Linguagem: Inglês

10.1002/mcs.1220020506

1520-667X

Hiroyuki Nishi, Tsukasa Fukuyama, Masaaki Matsuo,

Advanced Chemical Sensor Technologies

Abstract Resolution of isomers of 2,3,4,6‐tetra‐ O ‐acetyl‐β‐D‐glucopyranosyl isothiocyanate (GITC)–derivatized DL‐amino acids was accomplished using micellar electrokinetic chromatography with sodium dodecyl sulfate (SDS) solutions. These solutes were not separated by the usual capillary zone electrophoresis. Of 21 DL‐amino acids, optical resolution was recognized in 19 under the neutral and alkaline conditions; aspartic acid and glutamic acid have an additional carboxyl group. Simultaneous resolution of more than ten GITC derivatized DL‐amino acids was achieved within 40 min with the addition of 0.2 M SDS. The diastereomers of aromatic, heterocyclic, and basic DL‐amino acids were successfully separated owing to their effective solubilization into the SDS micelle, although a few peaks of the derivatives coeluted.