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Microsphere‐Based Real‐Time Calcium Sensing

2006; Wiley; Volume: 45; Issue: 33 Linguagem: Inglês

10.1002/anie.200601242

1521-3773

Rosario M. Sánchez‐Martín, Matt Cuttle, Stifun Mittoo, Mark Bradley,

Antimicrobial Peptides and Activities

Angewandte Chemie International EditionVolume 45, Issue 33 p. 5472-5474 Communication Microsphere-Based Real-Time Calcium Sensing† Rosario M. Sánchez-Martín Dr., Rosario M. Sánchez-Martín Dr. School of Chemistry, University of Edinburgh, Joseph Black building West Mains Road, Edinburgh EH9 3JJ, UK, Fax: (+44) 131-650-6453Search for more papers by this authorMatt Cuttle Dr., Matt Cuttle Dr. School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UKSearch for more papers by this authorStifun Mittoo Dr., Stifun Mittoo Dr. Activotec, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UKSearch for more papers by this authorMark Bradley Prof., Mark Bradley Prof. [email protected] School of Chemistry, University of Edinburgh, Joseph Black building West Mains Road, Edinburgh EH9 3JJ, UK, Fax: (+44) 131-650-6453Search for more papers by this author Rosario M. Sánchez-Martín Dr., Rosario M. Sánchez-Martín Dr. School of Chemistry, University of Edinburgh, Joseph Black building West Mains Road, Edinburgh EH9 3JJ, UK, Fax: (+44) 131-650-6453Search for more papers by this authorMatt Cuttle Dr., Matt Cuttle Dr. School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UKSearch for more papers by this authorStifun Mittoo Dr., Stifun Mittoo Dr. Activotec, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UKSearch for more papers by this authorMark Bradley Prof., Mark Bradley Prof. [email protected] School of Chemistry, University of Edinburgh, Joseph Black building West Mains Road, Edinburgh EH9 3JJ, UK, Fax: (+44) 131-650-6453Search for more papers by this author First published: 11 August 2006 https://doi.org/10.1002/anie.200601242Citations: 61 † This research was supported by the EPSRC. R.M.S.-M. thanks the Royal Society for a Dorothy Hodgkin Fellowship. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Sensing within the cell: Microspheres (2 μm) covalently loaded with calcium sensors can be delivered efficiently into living cells to analyze the intracellular changes in the concentration of Ca2+ ions in real time. The figure shows the real-time ratiometric fluorescent analysis (400/475 nm ratio) of the release of Ca2+ ions in cells loaded with the microspheres. References 1O. Eerbeek, E. G. Mik, C. J. Zuurbier, M. van't Loo, C. Donkersloot, C. Ince, J. Appl. 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Zuhorn, D. Hoeskstra, Biochem. J. 2004, 377, 159– 169. Citing Literature Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z601242_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume45, Issue33August 18, 2006Pages 5472-5474 ReferencesRelatedInformation