Triggered amplification by hybridization chain reaction
2004; National Academy of Sciences; Volume: 101; Issue: 43 Linguagem: Inglês
10.1073/pnas.0407024101
ISSN1091-6490
AutoresRobert M. Dirks, Niles A. Pierce,
Tópico(s)RNA Interference and Gene Delivery
ResumoWe introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.
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