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Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

1986; Cold Spring Harbor Laboratory Press; Volume: 51; Linguagem: Inglês

10.1101/sqb.1986.051.01.032

1943-4456

Kary B. Mullis, Fred Faloona, S. Scharf, Randall K. Saiki, Glenn T. Horn, M Kellis,

DNA and Nucleic Acid Chemistry

The discovery of specific restriction endonucleases (Smith and Wilcox 1970) made possible the isolation of discrete molecular fragments of naturally occurring DNA for the first time. This capability was crucial to the development of molecular cloning (Cohen et al. 1973); and the combination of molecular cloning and endonuclease restriction allowed the synthesis and isolation of any naturally occurring DNA sequence that could be cloned into a useful vector and, on the basis of flanking restriction sites, excised from it. The availability of a large variety of restriction enzymes (Roberts 1985) has significantly extended the utility of these methods.