
Hepatitis A virus subgenotypes dissemination during a community outbreak in a surrounding region of Rio de Janeiro
2008; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 103; Issue: 3 Linguagem: Inglês
10.1590/s0074-02762008000300005
ISSN1678-8060
AutoresDébora Regina Lopes dos Santos, Lívia Melo Villar, Vanessa Salete de Paula, Gerson Silva de Lima, Ana Gaspar,
Tópico(s)Viral Infections and Immunology Research
ResumoFrom December 1999 to December 2001, many cases of hepatitis A were notified in the county of Belford Roxo involving individuals aged 0 to 79 years. Serum samples were collected to evaluate the prevalence of anti-hepatitis A virus (HAV) antibodies, to detect HAV-RNA and to correlate with possible risk factors of HAV infection. Serum samples were screened by commercial IgM and total anti-HAV antibody ELISA and HAV-RNA was isolated and subsequently amplified by reverse transcription-polymerase chain reaction (RT-PCR) at VP1/2A region, sequenced and analyzed. Total anti-HAV prevalence was 87.9% (203/231) and IgM anti-HAV prevalence was 38.7% (89/231). Multivariate analysis showed that individuals under 20 years old are risks groups to acquire the infection suggesting that hygienic habits of young subjects are the principal factor of transmission and so they could be the target for vaccine programs. HAV-RNA was amplified from 29 (32.5%) IgM anti-HAV positive patients and 26 samples were sequenced and classified into subgenotypes IB (8 isolates) and IA (18 isolates). Isolates classified into subgenotype IB were identical representing one distinct strain. We could observe both subgenotypes circulating during the study which suggests different sources of infection. Prophylactic measures as vaccination strategies added to improvements in hygienic and sanitary conditions would be highly effective to reduction of infection.
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