Blood-brain barrier disruption highly induces aquaporin-4 mRNA and protein in perivascular and parenchymal astrocytes: Protective effect by estradiol treatment in ovariectomized animals
2005; Wiley; Volume: 80; Issue: 2 Linguagem: Inglês
10.1002/jnr.20443
ISSN1097-4547
AutoresMayka Tomás‐Camardiel, José L. Venero, Antonio J. Herrera, Rocío M. de Pablos, José A. Pintor‐Toro, Ausenda Machado, J. Cano,
Tópico(s)Barrier Structure and Function Studies
ResumoJournal of Neuroscience ResearchVolume 80, Issue 2 p. 235-246 Research Article Blood–brain barrier disruption highly induces aquaporin-4 mRNA and protein in perivascular and parenchymal astrocytes: Protective effect by estradiol treatment in ovariectomized animals M. Tomás-Camardiel, M. Tomás-Camardiel Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ.L. Venero, J.L. Venero Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorA.J. Herrera, A.J. Herrera Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorR.M. De Pablos, R.M. De Pablos Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ.A. Pintor-Toro, J.A. Pintor-Toro Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla, SpainSearch for more papers by this authorA. Machado, A. Machado Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ. Cano, Corresponding Author J. Cano josefina@us.es Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainDepartamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/ Prof. García González, 2, 41012-Sevilla, SpainSearch for more papers by this author M. Tomás-Camardiel, M. Tomás-Camardiel Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ.L. Venero, J.L. Venero Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorA.J. Herrera, A.J. Herrera Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorR.M. De Pablos, R.M. De Pablos Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ.A. Pintor-Toro, J.A. Pintor-Toro Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla, SpainSearch for more papers by this authorA. Machado, A. Machado Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainSearch for more papers by this authorJ. Cano, Corresponding Author J. Cano josefina@us.es Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainDepartamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/ Prof. García González, 2, 41012-Sevilla, SpainSearch for more papers by this author First published: 16 March 2005 https://doi.org/10.1002/jnr.20443Citations: 87Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Strong evidence involves aquaporin-4 (AQP4) in the physiopathology of brain edema. Two major points remain unsolved: (1) the capacity of perivascular glial cells to regulate AQP4 in response to disruption of the blood–brain barrier (BBB); and (2) the potential beneficial role of AQP4 in the clearance of brain edema. We used intraparenchymal injection of lipopolysaccharide (LPS) as an efficient model to induce BBB disruption. This was monitored by IgG extravasation and AQP4 was studied at the mRNA and protein level. The first signs of BBB disruption coincided with strong induction of AQP4 mRNA in perivascular glial cells. At the early phase, estradiol treatment highly prevented the LPS-induced disruption of the BBB and the induction of AQP4. Efficient clearance of vasogenic edema is supposed to occur once BBB is restored. This phase coincided with high induction of AQP4 mRNA in parenchymal reactive astrocytes and perivascular glial processes. High levels of AQP4 mRNA may be beneficial under these conditions. Our data may clarify why estradiol treatment reduces mortality in conditions typically associated with edema formation, like stroke. © 2005 Wiley-Liss, Inc. Citing Literature Volume80, Issue215 April 2005Pages 235-246 RelatedInformation
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