Low‐copy nuclear primers and ycf1 primers in Cactaceae
2012; Wiley; Volume: 99; Issue: 10 Linguagem: Inglês
10.3732/ajb.1200128
ISSN1537-2197
AutoresAlan R. Franck, Bruce J. Cochrane, James R. Garey,
Tópico(s)Plant and Fungal Species Descriptions
Resumo• Premise of the study: To increase the number of variable regions available for phylogenetic study in the Cactaceae, primers were developed for a portion of the plastid ycf1 gene and intron‐spanning regions of two low‐copy nuclear genes ( isi1 , nhx1 ). • Methods and Results: Primers were tested on several families within Caryophyllales, focusing on the Cactaceae. Gel electrophoresis indicated positive amplification in most samples. Sequences of these three regions ( isi1 , nhx1 , ycf1 ) from Harrisia exhibited variation similar to or greater than two plastid regions ( atpB‐rbcL intergenic spacer and rpl16 intron). • Conclusions: The isi , nhx , and ycf1 primers amplify phylogenetically useful information applicable to the Cactaceae and other families in the Caryophyllales.
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