Does Bilirubin Cause Interference in Roche Creatinine Methods?
2007; American Association for Clinical Chemistry; Volume: 53; Issue: 2 Linguagem: Inglês
10.1373/clinchem.2006.075846
ISSN1530-8561
Autores Tópico(s)Heme Oxygenase-1 and Carbon Monoxide
ResumoBilirubin interference in kinetic alkaline picrate (Jaffe) creatinine assays has been well documented (1)(2). The exact mechanism of interference is still unclear, however, and both conjugated and unconjugated bilirubin have been implicated. Enzymatic assays, which reportedly suffer less interference, are an alternative, but the extra reagent cost has restricted their use. Historical data continue to be used to estimate the extent of bilirubin interference, although modifications to these assays or instruments may have taken place since the original interference studies were performed. We used liquid chromatography tandem mass spectrometry (LC-MS/MS) as the reference method to investigate the extent of bilirubin interference in 2 automated creatinine assays. We have previously shown that this LC-MS/MS assay compares well to the 2 automated methods at creatinine concentrations of 100 samples with bilirubin concentrations within reference intervals. We added varying amounts of creatinine (Sigma) to phosphate buffered saline (PBS) pH 7.4, containing 40 g/L bovine serum albumin, to give concentrations of 37.5–1000 μmol/L. One liter of PBS contained 8 g sodium chloride, 0.2 …
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