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Artigo Revisado por pares
BIBTEX RIS

Regulation of heat shock protein synthesis in rat astroeytes

1991; Wiley; Volume: 28; Issue: 3 Linguagem: Inglês

10.1002/jnr.490280306

ISSN

1097-4547

Autores

Barney E. Dwyer, Robert N. Nishimura, Jean de Vellis, Kerry B. Clegg,

Tópico(s)

Physiological and biochemical adaptations

Resumo

Abstract Rat forebrain astrocytes synthesize heat shock proteins with molecular weights 97,89,70,68, and 30‐34 kilodaltons. The stress inducible 68‐kDa heat shock protein (HSP‐68) was vigorously expressed by astro‐cytes in culture after a 45°C, 20 min heat shock. HSP‐68 synthesis was poorly inducible by a second heat shock given 16 hr after the initial heat shock. Decreased [ 35 S]methionine incorporation into HSP‐68 correlated with low levels of HSP‐68 mRNA present after the second heat shock. The data suggest that control of HSP‐68 mRNA levels by transcriptional/ posttranscriptional mechanisms is a major site for regulation of HSP‐68 synthesis.

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