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Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

2013; Royal Society of Chemistry; Volume: 5; Issue: 20 Linguagem: Inglês

10.1039/c3nr03683a

2040-3372

Simon Cabello-Aguilar, Sébastien Balme, Adib Abou Chaaya, Mikhaël Bechelany, E. Balanzat, Jean‐Marc Janot, Céline Pochat‐Bohatier, Philippe Miele, Philippe Déjardin,

Electrostatics and Colloid Interactions

We report the formation of a hybrid biological/artificial nanopore by the direct insertion of non-modified α-hemolysin at the entrance of a high aspect ratio (length/diameter) biomimetic nanopore. In this robust hybrid system, the protein exhibits the same polynucleotide discrimination properties as in the biological membrane and the polynucleotide dwell time is strongly increased. This nanopore is very promising for DNA sequencing applications where the high DNA translocation velocity and the fragility of the support are the main bottlenecks.