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A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing

2007; Cell Press; Volume: 129; Issue: 7 Linguagem: Inglês

10.1016/j.cell.2007.04.040

1097-4172

Pablo Landgraf, Mirabela Rusu, Robert P. Sheridan, Alain Sewer, Nicola Iovino, Alexei A. Aravin, Sébastien Pfeffer, Amanda Rice, Alice O. Kamphorst, Markus Landthaler, Carolina Lin, Nicholas D. Socci, Leandro C. Hermida, Valerio Fulci, Sabina Chiaretti, Robin Foà, Julia Schliwka, Uta Fuchs, Astrid Novosel, Roman‐Ulrich Müller, Bernhard Schermer, Ute Bissels, Jason Inman, Quang N. Phan, Minchen Chien, David B. Weir, Ruchi Choksi, Gabriella De Vita, Daniela Frezzetti, Hans‐Ingo Trompeter, Veit Hornung, Grace Teng, Gunther Hartmann, Miklós Palkovits, Roberto Di Lauro, Peter Wernet, Giuseppe Macino, Charles E. Rogler, James W. Nagle, Jingyue Ju, F. Nina Papavasiliou, Thomas Benzing, Peter Lichter, Wayne Tam, Michael Brownstein, Andreas Bosio, Arndt Borkhardt, James J. Russo, Chris Sander, Mihaela Zavolan, Thomas Tuschl,

RNA Research and Splicing

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.