Nucleotide sequences of alcohol acetyltransferase genes from lager brewing yeast, Saccharomyces carlsbergensis
1996; Wiley; Volume: 12; Issue: 6 Linguagem: Inglês
10.1002/(sici)1097-0061(199605)12
ISSN1097-0061
AutoresToshio Fujii, Hiroyuki Yoshimoto, Naoshi Nagasawa, Takayuki Bogaki, Yukio Tamai, Masaaki Hamachi,
Tópico(s)Microbial Metabolic Engineering and Bioproduction
ResumoThe nucleotide sequences of alcohol acetyltransferase genes isolated from lager brewing yeast, Saccharomyces carlsbergensis have been determined. S. carlsbergensis has one ATF1 gene and another homologous gene, the Lg-ATF1 gene. There was a high degree of homology between the amino acid sequences deduced for the ATF1 protein and the Lg-ATF1 protein (75.7%), but the N-terminal region has a relatively low degree of homology. Southern analysis and contour-clamped homogeneous electric field analysis of Saccharomyces strains suggest that the ATF1 gene is located on chromosome XV in S. cerevisiae and that the Lg-ATF1 gene might originate from the "non-S. cerevisiae' genome of S. carlsbergensis, which is similar to that of S. bayanus and S. pastorianus.
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