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Determination of Pseudomonas aeruginosa by Biochemical Test Methods

1970; Wiley; Volume: 14; Issue: 1 Linguagem: Inglês

10.1111/j.1348-0421.1970.tb00490.x

0021-5139

Taketoshi Arai, Seiji Enomoto, Shōgo Kuwahara,

Plant Pathogenic Bacteria Studies

ABSTRACT A new test method was devised for microbial gluconate oxidation, using an ammonium molybdate reagent. One loopful (about 2 mg wet wt.) of the test organism, grown on a nutrient agar plate for 18 hr, is transferred into 1 ml of the test liquid medium consisting of (NH 4 ) 2 SO 4 0.5 mg, potassium gluconate 10 mg, NaCl 5 mg, KH 2 PO 4 2 mg, MgSO 4 ·7H 2 O 0.1 mg, and 1 ml of distilled water, incubated at 37 C for 6 hr without shaking, and then mixed with 3 ml of 1% aqueous solution of ammonium molybdate and 0.2 ml of glacial acetic acid. The mixture is heated in boiling water for 5 min, followed by abrupt cooling with running water. A deep blue colour appears in a positive result. A total of 39 strains of Pseudomonas aeruginosa showed positive results by this method, whereas Aeromonas, Vibrio, Proteus group, Klebsiella, Citrobacter and Enterobacter A group were all negative. Though some strains of Enterobacter B group showed a weak blue colour, it could be easily differentiated from the deep blue colour of Pseudomonas . Longer incubation of test microbes in the test medium, and longer heating of the reaction mixture gave unsatisfactory results.