Portal de Periódicos da CAPES

Design and Performance of a 16S rRNA-Targeted Oligonucleotide Probe for Detection of Members of the Genus Bdellovibrio by Fluorescence In Situ Hybridization

2007; American Society for Microbiology; Volume: 73; Issue: 22 Linguagem: Inglês

10.1128/aem.01112-07

1098-5336

Khaled K. Mahmoud, Damian McNeely, Chelsea Elwood, Susan F. Koval,

Microbial Community Ecology and Physiology

ABSTRACT A 16S rRNA-targeted, Cy3-labeled oligonucleotide probe was designed to detect members of the genus Bdellovibrio by fluorescence in situ hybridization. Specific hybridization conditions were established; however, the detection of bdellovibrios in environmental samples required enrichment, confirming that Bdellovibrio spp. are not present in large numbers in the environment.