Produção Nacional
Revisado por pares
Determination of triamcinolone in human plasma by a sensitive HPLC–ESI‐MS/MS method: application for a pharmacokinetic study using nasal spray formulation
2011; Wiley; Volume: 46; Issue: 3 Linguagem: Inglês
10.1002/jms.1896
ISSN1096-9888
AutoresIsabela Costa César, Ricardo Martins Duarte Byrro, Fabiana Fernandes de Santana e Silva Cardoso, Iram Moreira Mundim, Leonardo de Souza Teixeira, Weidson Carlo de Sousa, Sandro Antônio Gomes, Karini Bruno Bellório, Juliana Machado Brêtas, Gérson Antônio Pianetti,
Tópico(s)Antibiotics Pharmacokinetics and Efficacy
ResumoA liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55:45). Selected reaction monitoring was performed using the transitions m/z 435 → 415 and m/z 393 → 373 to quantify triamcinolone acetonide and betamethasone, respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for triamcinolone acetonide. The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of triamcinolone acetonide in healthy volunteers, in a pharmacokinetic study with nasal spray formulation.
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