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Evidence for evolution of canine parvovirus type 2 in Italy

2001; Microbiology Society; Volume: 82; Issue: 12 Linguagem: Inglês

10.1099/0022-1317-82-12-3021

1465-2099

Canio Buonavoglia, Vito Martella, Annamaria Pratelli, Maria Tempesta, Alessandra Cavalli, Domenico Buonavoglia, Giancarlo Bozzo, Gabriella Elia, Nicola Decaro, L. E. Carmichael,

Animal Virus Infections Studies

Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay.