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Reverse Transcriptase Activity in Mature Spermatozoa of Mouse

2000; Rockefeller University Press; Volume: 148; Issue: 6 Linguagem: Inglês

10.1083/jcb.148.6.1107

1540-8140

Roberto Giordano, Anna Rosa Magnano, Germana Zaccagnini, Carmine Pittoggi, N Moscufo, R Lorenzini, Corrado Spadafora,

Viral gastroenteritis research and epidemiology

We show here that a reverse transcriptase (RT) activity is present in murine epididymal spermatozoa. Sperm cells incubated with human poliovirus RNA can take up exogenous RNA molecules and internalize them in nuclei. Direct PCR amplification of DNA extracted from RNA-incubated spermatozoa indicate that poliovirus RNA is reverse-transcribed in cDNA fragments. PCR analysis of two-cell embryos shows that poliovirus RNA-challenged spermatozoa transfer retrotranscribed cDNA molecules into eggs during in vitro fertilization. Finally, RT molecules can be visualized on sperm nuclear scaffolds by immunogold electron microscopy. These results, therefore, reveal a novel metabolic function in spermatozoa, which may play a role during early embryonic development.