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Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging

2003; Optica Publishing Group; Volume: 20; Issue: 2 Linguagem: Inglês

10.1364/josaa.20.000368

1520-8532

Johan Philip, Kjell Carlsson,

Advanced Fluorescence Microscopy Techniques

We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice of detection technique and its parameters is important for obtaining good results.