Produção Nacional
Revisado por pares
Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats
2012; Wiley; Volume: 222; Issue: 2 Linguagem: Inglês
10.1111/joa.12009
ISSN1469-7580
AutoresGustavo Orione Puntel, Nélson Rodrigues de Carvalho, Fernando Dobrachinski, Andréia Caroline Fernandes Salgueiro, Robson Luiz Puntel, Vanderlei Folmer, Nilda Vargas Barbosa, Luiz Fernando Freire Royes, João Batista Teixeira da Rocha, Félix Alexandre Antunes Soares,
Tópico(s)Medical and Biological Ozone Research
ResumoAbstract The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused ( I / R ) gastrocnemius muscle of rats. Forty male W istar rats were divided into control and I / R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid‐reactive substances and dichlorofluorescein oxidation levels were observed in I / R muscle. Cryotherapy in I / R muscle also minimized effects such as decreased cellular viability, levels of non‐protein thiols and calcium ATP ase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I / R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I / R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction.
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