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Association of a NOD2 Gene Polymorphism and T-Helper 17 Cells With Presumed Ocular Toxoplasmosis

2012; Oxford University Press; Volume: 207; Issue: 1 Linguagem: Inglês

10.1093/infdis/jis640

1537-6613

Míriam Santos Dutra, Samantha Ribeiro Béla, Alba Lucínia Peixoto Rangel, Michaela Fakiola, A. G. Cruz, Andréa Gazzinelli, Humberto Ferreira Oliveira Quites, Lilian M. G. Bahia‐Oliveira, Ricardo Guerra Peixe, Wagner Reis da Costa Campos, A. C. Higino-Rocha, Nicole E. Miller, Jenefer M. Blackwell, Lis Ribeiro do Valle Antonelli, Ricardo T. Gazzinelli,

Inflammasome and immune disorders

Retinochoroiditis manifests in patients infected with Toxoplasma gondii. Here, we assessed 30 sibships and 89 parent/case trios of presumed ocular toxoplasmosis (POT) to evaluate associations with polymorphisms in the NOD2 gene. Three haplotype-tagging single-nucleotide polymorphisms (tag-SNPs) within the NOD2 gene were genotyped. The family-based association test showed that the tag-SNP rs3135499 is associated with retinochoroiditis (P = .039). We then characterized the cellular immune response of 59 cases of POT and 4 cases of active ocular toxoplasmosis (AOT). We found no differences in levels of interferon γ (IFN-γ) and interleukin 2 produced by T-helper 1 cells when comparing patients with AOT or POT to asymptomatic individuals. Unexpectedly, we found an increased interleukin 17A (IL-17A) production in patients with POT or OAT. In patients with POT or AOT, the main cellular source of IL-17A was CD4+CD45RO+T-bet−IFN-γ− T-helper 17 cells. Altogether, our results suggest that NOD2 influences the production of IL-17A by CD4+ T lymphocytes and might contribute to the development of ocular toxoplasmosis.