Portal de Periódicos da CAPES

Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery

2008; Elsevier BV; Volume: 130; Issue: 2 Linguagem: Inglês

10.1016/j.jconrel.2008.05.028

1873-4995

Raquel Mejías, R. Costo, Alejandro G. Roca, Cristina Fernańdez-Arias, S. Veintemillas‐Verdaguer, T. González-Carreño, M. P. Morales, Carlos J. Serna, Santos Mañes, Domingo F. Barber,

Immunotherapy and Immune Responses

Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-γ) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation , decomposition in organic media, and laser pyrolysis. To facilitate IFN-γ adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-γ and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-γ adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.