Voltar
Artigo Acesso aberto Revisado por pares
BIBTEX RIS

Inducible-Expression Plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans

2009; American Society for Microbiology; Volume: 75; Issue: 20 Linguagem: Inglês

10.1128/aem.01587-09

ISSN

1098-5336

Autores

Alice C. Ind, Steven L. Porter, Mostyn T. Brown, Elaine D. Byles, Jennifer Anne de Beyer, Scott A. C. Godfrey, Judith P. Armitage,

Tópico(s)

Enzyme Structure and Function

Resumo

We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.

Referência(s)