Expression of green fluorescent protein in Lactococcus lactis
2000; Oxford University Press; Volume: 183; Issue: 2 Linguagem: Inglês
10.1111/j.1574-6968.2000.tb08963.x
ISSN1574-6968
AutoresPilar Fernández de Palencia, Concha Nieto, Paloma Acebo, Manuel Espinosa, Paloma Là pez,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoThe gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. lactis biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored in living cells during growth to evaluate its use in molecular and physiological studies. Quantification of the levels of GFP expressed by cultures was feasible by fluorescence spectroscopy. Phase-contrast and fluorescence microscopy allowed us to distinguish, in mixed cultures, lactococcal cells expressing GFP. Our results indicate that GFP can be used as a reporter in L. lactis.
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