Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentrations during metabolic studies of very low density lipoprotein and low density lipoprotein apolipoprotein B.
1983; Elsevier BV; Volume: 24; Issue: 9 Linguagem: Inglês
10.1016/s0022-2275(20)37908-6
ISSN1539-7262
AutoresGenshi Egusa, David Brady, Scott M. Grundy, Barbara V. Howard,
Tópico(s)Lipoproteins and Cardiovascular Health
ResumoA method has been described for the measurement of apoB concentration and specific activity in very low density lipoprotein (VLDL) and low density lipoprotein (LDL) during metabolic studies.For measurement of specific activity, apoB was separated from other apolipoproteins and lipids by precipitation in, and subsequent washing with, isopropanol.For determination of apoB concentration, equal volumes of lipoprotein and isopropanol were mixed, and the protein content of the apoB precipitate was measured by the difference between total lipoprotein protein and the protein measured in the supernatant.Evidence that there was no apoB solubilization in isopropanol and that precipitated apoB was virtually free of soluble apolipoproteins was obtained by electrophoresis.Lipid contamination of the apoB precipitate was less than
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